This Article Full Text Full Text (PDF) All Versions of this Article: 139/4/1914    most recent pp.105.066795v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Katou, S. Articles by Doke, N. Search for Related Content PubMed PubMed Citation Articles by Katou, S. Articles by Doke, N. Agricola Articles by Katou, S. Articles by Doke, N.

PLANTS INTERACTING WITH OTHER ORGANISMS

Involvement of PPS3 Phosphorylated by Elicitor-Responsive Mitogen-Activated Protein Kinases in the Regulation of Plant Cell Death¹

Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464–8601, Japan (S.K., H.Y., K.K., N.D.); Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom (O.R., J.D.G.J.); and Developmental Regulation Laboratory, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464–8601, Japan (H.M.)

Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in plant innate immunity. Overexpression of StMEK1^DD, a constitutively active MAPK kinase that activates salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), provokes hypersensitive response-like cell death in Nicotiana benthamiana. Here we purified a 51-kD MAPK, which was activated in potato (Solanum tuberosum) tubers treated with hyphal wall elicitor of a plant pathogen, and isolated the cDNA designated StMPK1. The deduced amino acid sequence of the StMPK1 showed strong similarity to stress-responsive MAPKs, such as tobacco (Nicotiana tabacum) SIPK and Arabidopsis (Arabidopsis thaliana) AtMPK6. To investigate the downstream signaling of StMPK1, we identified several proteins phosphorylated by StMPK1 (PPSs) using an in vitro expression cloning method. To dissect the biological function of PPSs in the plant defense, we employed virus-induced gene silencing (VIGS) in N. benthamiana. VIGS of NbPPS3 significantly delayed cell death induced by the transient expression of StMEK1^DD and treatment with hyphal wall elicitor. Furthermore, the mobility shift of NbPPS3 on SDS-polyacrylamide gel was induced by transient expression of StMEK1^DD. The mobility shift of NbPPS3 induced by StMEK1^DD was not compromised by VIGS of WIPK or SIPK alone, but drastically reduced by the silencing of both WIPK and SIPK. This work strongly supports the idea that PPS3 is a physiological substrate of StMPK1 and is involved in cell death activated by a MAPK cascade.

² Present address: Department of Biology, Carleton University, Ottawa, Ontario, Canada K1S 5B6.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Hirofumi Yoshioka (hyoshiok{at}agr.nagoya-u.ac.jp).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.066795.

^* Corresponding author; e-mail hyoshiok{at}agr.nagoya-u.ac.jp; fax: 81–52–789–5525.

Received June 6, 2005; returned for revision September 11, 2005; accepted October 10, 2005.

This article has been cited by other articles:

				L. Katsir, A. L. Schilmiller, P. E. Staswick, S. Y. He, and G. A. Howe From the Cover: COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine PNAS, May 13, 2008; 105(19): 7100 - 7105. [Abstract] [Full Text] [PDF]

				S. Asai, K. Ohta, and H. Yoshioka MAPK Signaling Regulates Nitric Oxide and NADPH Oxidase-Dependent Oxidative Bursts in Nicotiana benthamiana PLANT CELL, May 1, 2008; 20(5): 1390 - 1406. [Abstract] [Full Text] [PDF]

				K. Kazan and J. M. Manners Jasmonate Signaling: Toward an Integrated View Plant Physiology, April 1, 2008; 146(4): 1459 - 1468. [Full Text] [PDF]

				R. Takabatake, Y. Ando, S. Seo, S. Katou, S. Tsuda, Y. Ohashi, and I. Mitsuhara MAP Kinases Function Downstream of HSP90 and Upstream of Mitochondria in TMV Resistance Gene N-Mediated Hypersensitive Cell Death Plant Cell Physiol., March 1, 2007; 48(3): 498 - 510. [Abstract] [Full Text] [PDF]

				M. Kobayashi, I. Ohura, K. Kawakita, N. Yokota, M. Fujiwara, K. Shimamoto, N. Doke, and H. Yoshioka Calcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH Oxidase PLANT CELL, March 1, 2007; 19(3): 1065 - 1080. [Abstract] [Full Text] [PDF]

				H.-C. Lu, H.-H. Chen, W.-C. Tsai, W.-H. Chen, H.-J. Su, D. C.-N. Chang, and H.-H. Yeh Strategies for Functional Validation of Genes Involved in Reproductive Stages of Orchids Plant Physiology, February 1, 2007; 143(2): 558 - 569. [Abstract] [Full Text] [PDF]

				C. Yamamizo, K. Kuchimura, A. Kobayashi, S. Katou, K. Kawakita, J. D.G. Jones, N. Doke, and H. Yoshioka Rewiring Mitogen-Activated Protein Kinase Cascade by Positive Feedback Confers Potato Blight Resistance Plant Physiology, February 1, 2006; 140(2): 681 - 692. [Abstract] [Full Text] [PDF]