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GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Ecotype Allelic Variation in C-to-U Editing Extent of a Mitochondrial Transcript Identifies RNA-Editing Quantitative Trait Loci in Arabidopsis¹

Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853

In higher plants, RNA editing is a posttranscriptional process that converts C to U in organelle mRNAs. Although RNA editing in mitochondria occurs much more frequently than in chloroplasts, editing of exogenously supplied RNA substrates in vitro and in organello has shown that editing in the two organelles shares some common features. In particular, the 20 nucleotides upstream of the editing site play an important role in specifying the C to be edited. Biochemical approaches have allowed the identification of features of cis-sequences necessary for RNA editing to occur, but have failed to identify any of the components of the mitochondrial editing machinery. In order to implement a genetic approach for identification of editing factors, we have identified a polymorphism in the editing efficiency of a mitochondrial site between two ecotypes of Arabidopsis (Arabidopsis thaliana), Columbia (Col) and Landsberg erecta (Ler). In rosette leaves, an editing site within the ccb206 mitochondrial gene is more highly edited in Col than in Ler. Depending on the development stage and tissue analyzed, the difference in editing extent varies between the two ecotypes; for example, in floral buds, editing extent does not differ. Single-point regression analysis of the editing efficiency in a sample of recombinant inbred lines derived from a cross between Col and Ler allowed the identification of two quantitative trait loci controlling this trait. A member of the pentatricopeptide repeat protein family that carries a putative mitochondrial transit sequence has been identified near a major quantitative trait locus on chromosome 4.

² These authors contributed equally to the paper.

³ Present address: Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique (INRA)/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique, "Genomique et Biotechnologie des Fruits," Centre INRA Toulouse Auzeville, Chemin de Borde Rouge, 31320 Castanet-Tolosan, France.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Stéphane Bentolila (sb46{at}cornell.edu).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.069013.

^* Corresponding author; e-mail mrh5{at}cornell.edu; fax 607–255–6249.

Received July 27, 2005; returned for revision September 13, 2005; accepted September 23, 2005.

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