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First published online December 16, 2005; 10.1104/pp.105.066464 Plant Physiology 140:81-90 (2006) © 2006 American Society of Plant Biologists Lectin Receptor Kinases Participate in Protein-Protein Interactions to Mediate Plasma Membrane-Cell Wall Adhesions in Arabidopsis1Surfaces Cellulaires et Signalisation chez les Végétaux, Unité Mixte de Recherche 5546 Centre National de la Recherche Scientifique-Université Paul Sabatier, 31326 Castanet Tolosan, France (A.G., V.S., A.S., A.B., P.R., R.P.-L., H.C.); and Laboratory of Phytopathology, Plant Sciences Group, Wageningen University, 6700 EE Wageningen, The Netherlands (V.S., F.G.)
Interactions between plant cell walls and plasma membranes are essential for cells to function properly, but the molecules that mediate the structural continuity between wall and membrane are unknown. Some of these interactions, which are visualized upon tissue plasmolysis in Arabidopsis (Arabidopsis thaliana), are disrupted by the RGD (arginine-glycine-aspartic acid) tripeptide sequence, a characteristic cell adhesion motif in mammals. In planta induced-O (IPI-O) is an RGD-containing protein from the plant pathogen Phytophthora infestans that can disrupt cell wall-plasma membrane adhesions through its RGD motif. To identify peptide sequences that specifically bind the RGD motif of the IPI-O protein and potentially play a role in receptor recognition, we screened a heptamer peptide library displayed in a filamentous phage and selected two peptides acting as inhibitors of the plasma membrane RGD-binding activity of Arabidopsis. Moreover, the two peptides also disrupted cell wall-plasma membrane adhesions. Sequence comparison of the RGD-binding peptides with the Arabidopsis proteome revealed 12 proteins containing amino acid sequences in their extracellular domains common with the two RGD-binding peptides. Eight belong to the receptor-like kinase family, four of which have a lectin-like extracellular domain. The lectin domain of one of these, At5g60300, recognized the RGD motif both in peptides and proteins. These results imply that lectin receptor kinases are involved in protein-protein interactions with RGD-containing proteins as potential ligands, and play a structural and signaling role at the plant cell surfaces.
1 This work was supported by the Université Paul Sabatier, Toulouse; by the Centre National de la Recherche Scientifique; by GABI/Génoplante (contract no. AF–2001091); and by the Netherlands-French bilateral exchange program Van Gogh (NWO-VGP 85–343). 2 These authors contributed equally to the paper. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Rafael Pont-Lezica (lezica@scsv.ups-tlse.fr). Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.066464. * Corresponding author; e-mail lezica{at}scsv.ups-tlse.fr; fax 33–562–19–35–02. Received June 2, 2005; returned for revision October 26, 2005; accepted November 15, 2005. This article has been cited by other articles:
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