This Article Full Text Full Text (PDF) All Versions of this Article: 140/1/91    most recent pp.105.071670v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Wang, X. Articles by Chong, K. Search for Related Content PubMed PubMed Citation Articles by Wang, X. Articles by Chong, K. Agricola Articles by Wang, X. Articles by Chong, K.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Overexpression of RAN1 in Rice and Arabidopsis Alters Primordial Meristem, Mitotic Progress, and Sensitivity to Auxin¹

Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China (X.W., Y.X., Y.H., Z.X., K.C.); Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China (S.B.); State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China (J.D., M.Y.); and Graduate School of the Chinese Academy of Sciences, Beijing 100049, China (X.W.)

Ran is an evolutionarily conserved eukaryotic GTPase. We previously identified a cDNA of TaRAN1, a novel Ran GTPase homologous gene in wheat (Triticum aestivum) and demonstrated that TaRAN1 is associated with regulation of genome integrity and cell division in yeast (Saccharomyces cerevisiae) systems. However, much less is known about the function of RAN in plant development. To analyze the possible biological roles of Ran GTPase, we overexpressed TaRAN1 in transgenic Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa). TaRAN1 overexpression increased the proportion of cells in the G2 phase of the cell cycle, which resulted in an elevated mitotic index and prolonged life cycle. Furthermore, it led to increased primordial tissue, reduced number of lateral roots, and stimulated hypersensitivity to exogenous auxin. The results suggest that Ran protein was involved in the regulation of mitotic progress, either in the shoot apical meristem or the root meristem zone in plants, where auxin signaling is involved. This article determines the function of RAN in plant development mediated by the cell cycle and its novel role in meristem initiation mediated by auxin signaling.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Kang Chong (chongk{at}ibcas.ac.cn).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.071670.

^* Corresponding author; e-mail chongk{at}ibcas.ac.cn; fax 86–10–82594821.

Received September 16, 2005; returned for revision November 16, 2005; accepted November 16, 2005.

This article has been cited by other articles:

				A. Zang, X. Xu, S. Neill, and W. Cai Overexpression of OsRAN2 in rice and Arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress J. Exp. Bot., March 1, 2010; 61(3): 777 - 789. [Abstract] [Full Text] [PDF]

				J. G. Dubrovsky, A. Soukup, S. Napsucialy-Mendivil, Z. Jeknic, and M. G. Ivanchenko The lateral root initiation index: an integrative measure of primordium formation Ann. Bot., March 1, 2009; 103(5): 807 - 817. [Abstract] [Full Text] [PDF]

				Y. Lee, H.-S. Lee, J.-S. Lee, S.-K. Kim, and S.-H. Kim Hormone- and light-regulated nucleocytoplasmic transport in plants: current status J. Exp. Bot., September 1, 2008; 59(12): 3229 - 3245. [Abstract] [Full Text] [PDF]

				K. Yoshimura, A. Masuda, M. Kuwano, A. Yokota, and K. Akashi Programmed Proteome Response for Drought Avoidance/Tolerance in the Root of a C3 Xerophyte (Wild Watermelon) Under Water Deficits Plant Cell Physiol., February 1, 2008; 49(2): 226 - 241. [Abstract] [Full Text] [PDF]

				M. M. Barthet and K. W. Hilu Expression of matK: functional and evolutionary implications Am. J. Botany, August 1, 2007; 94(8): 1402 - 1412. [Abstract] [Full Text] [PDF]

				I. Meier Composition of the plant nuclear envelope: theme and variations J. Exp. Bot., January 1, 2007; 58(1): 27 - 34. [Abstract] [Full Text] [PDF]