First published online January 11, 2006; 10.1104/pp.105.073676
Plant Physiology 140:771-778 (2006)
© 2006 American Society of Plant Biologists
WHOLE PLANT AND ECOPHYSIOLOGY
Evidence for Involvement of Photosynthetic Processes in the Stomatal Response to CO21
Susanna M. Messinger,
Thomas N. Buckley2 and
Keith A. Mott*
Biology Department, Utah State University, Logan, Utah 84322–5305 (S.M.M., K.A.M.); and Environmental Biology Group and Cooperative Research Centre for Greenhouse Accounting, Research School of Biological Sciences, Australian National University, Canberra, Australian Capital Territory 2601, Australia (T.N.B.)
Stomatal conductance (gs) typically declines in response to increasing intercellular CO2 concentration (ci). However, the mechanisms underlying this response are not fully understood. Recent work suggests that stomatal responses to ci and red light (RL) are linked to photosynthetic electron transport. We investigated the role of photosynthetic electron transport in the stomatal response to ci in intact leaves of cocklebur (Xanthium strumarium) plants by examining the responses of gs and net CO2 assimilation rate to ci in light and darkness, in the presence and absence of the photosystem II inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and at 2% and 21% ambient oxygen. Our results indicate that (1) gs and assimilation rate decline concurrently and with similar spatial patterns in response to DCMU; (2) the response of gs to ci changes slope in concert with the transition from Rubisco- to electron transport-limited photosynthesis at various irradiances and oxygen concentrations; (3) the response of gs to ci is similar in darkness and in DCMU-treated leaves, whereas the response in light in non-DCMU-treated leaves is much larger and has a different shape; (4) the response of gs to ci is insensitive to oxygen in DCMU-treated leaves or in darkness; and (5) stomata respond normally to RL when ci is held constant, indicating the RL response does not require a reduction in ci by mesophyll photosynthesis. Together, these results suggest that part of the stomatal response to ci involves the balance between photosynthetic electron transport and carbon reduction either in the mesophyll or in guard cell chloroplasts.
1 This work was supported by the National Science Foundation (grant no. 0416600 to K.A.M.) and by the Cooperative Research Centre for Greenhouse Accounting at the Research School of Biological Sciences, Australian National University (T.N.B.).
2 Present address: Biology Department, Utah State University, Logan, UT 84322–5305.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Keith A. Mott (kmott{at}biology.usu.edu).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.073676.
* Corresponding author; e-mail kmott{at}biology.usu.edu; fax 435–797–1575.
Received November 1, 2005;
returned for revision November 18, 2005;
accepted November 20, 2005.
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