This Article Full Text Full Text (PDF) All Versions of this Article: 140/3/1047    most recent pp.105.074955v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Lunkenbein, S. Articles by Schwab, W. Search for Related Content PubMed PubMed Citation Articles by Lunkenbein, S. Articles by Schwab, W. Agricola Articles by Lunkenbein, S. Articles by Schwab, W.

BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Cinnamate Metabolism in Ripening Fruit. Characterization of a UDP-Glucose:Cinnamate Glucosyltransferase from Strawberry¹

Biomolecular Food Technology, Technical University Munich, 85354 Freising, Germany (S.L., H.A.C., W.S.); Departamento de Bioquímica y Biología Molecular Edificio Severo Ochoa (C-6), Campus Universitario de Rabanales, Universidad de Córdoba, 14071 Cordoba, Spain (M.B., J.M.-B.); Plant Research International, Business Units Cell Cybernetics and Genetics and Breeding, 6700 AA Wageningen, The Netherlands (A.A., E.M.J.S.); and Section of Biology and Membrane Physics, Technical University Darmstadt, 64287 Darmstadt, Germany (R.K.)

Strawberry (Fragaria x ananassa) fruit accumulate (hydroxy)cinnamoyl glucose (Glc) esters, which may serve as the biogenetic precursors of diverse secondary metabolites, such as the flavor constituents methyl cinnamate and ethyl cinnamate. Here, we report on the isolation of a cDNA encoding a UDP-Glc:cinnamate glucosyltransferase (Fragaria x ananassa glucosyltransferase 2 [FaGT2]) from ripe strawberry cv Elsanta that catalyzes the formation of 1-O-acyl-Glc esters of cinnamic acid, benzoic acid, and their derivatives in vitro. Quantitative real-time PCR analysis indicated that FaGT2 transcripts accumulate to high levels during strawberry fruit ripening and to lower levels in flowers. The levels in fruits positively correlated with the in planta concentration of cinnamoyl, p-coumaroyl, and caffeoyl Glc. In the leaf, high amounts of Glc esters were detected, but FaGT2 mRNA was not observed. The expression of FaGT2 is negatively regulated by auxin, induced by oxidative stress, and by hydroxycinnamic acids. Although FaGT2 glucosylates a number of aromatic acids in vitro, quantitative analysis in transgenic lines containing an antisense construct of FaGT2 under the control of the constitutive 35S cauliflower mosaic virus promoter demonstrated that the enzyme is only involved in the formation of cinnamoyl Glc and p-coumaroyl Glc during ripening.

² These authors contributed equally to the paper.

³ Present address: Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Wilfried Schwab (schwab{at}wzw.tum.de).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.074955.

^* Corresponding author; e-mail schwab{at}wzw.tum.de; fax 49–8161–548–595.

Received November 30, 2005; returned for revision November 30, 2005; accepted December 14, 2005.

This article has been cited by other articles:

				S. Encinas-Villarejo, A. M. Maldonado, F. Amil-Ruiz, B. de los Santos, F. Romero, F. Pliego-Alfaro, J. Munoz-Blanco, and J. L. Caballero Evidence for a positive regulatory role of strawberry (Fragariaxananassa) Fa WRKY1 and Arabidopsis At WRKY75 proteins in resistance J. Exp. Bot., May 21, 2009; (2009) erp152v1. [Abstract] [Full Text] [PDF]

				A. Fait, K. Hanhineva, R. Beleggia, N. Dai, I. Rogachev, V. J. Nikiforova, A. R. Fernie, and A. Aharoni Reconfiguration of the Achene and Receptacle Metabolic Networks during Strawberry Fruit Development Plant Physiology, October 1, 2008; 148(2): 730 - 750. [Abstract] [Full Text] [PDF]

				M. Griesser, F. Vitzthum, B. Fink, M. L. Bellido, C. Raasch, J. Munoz-Blanco, and W. Schwab Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragariaxananassa) achene and receptacle J. Exp. Bot., July 1, 2008; 59(10): 2611 - 2625. [Abstract] [Full Text] [PDF]

				M. Griesser, T. Hoffmann, M. L. Bellido, C. Rosati, B. Fink, R. Kurtzer, A. Aharoni, J. Munoz-Blanco, and W. Schwab Redirection of Flavonoid Biosynthesis through the Down-Regulation of an Anthocyanidin Glucosyltransferase in Ripening Strawberry Fruit Plant Physiology, April 1, 2008; 146(4): 1528 - 1539. [Abstract] [Full Text] [PDF]

				J. Kapteyn, A. V. Qualley, Z. Xie, E. Fridman, N. Dudareva, and D. R. Gang Evolution of Cinnamate/p-Coumarate Carboxyl Methyltransferases and Their Role in the Biosynthesis of Methylcinnamate PLANT CELL, October 1, 2007; 19(10): 3212 - 3229. [Abstract] [Full Text] [PDF]

				S. Lunkenbein, E. M. J. Salentijn, H. A. Coiner, M. J. Boone, F. A. Krens, and W. Schwab Up- and down-regulation of Fragariaxananassa O-methyltransferase: impacts on furanone and phenylpropanoid metabolism J. Exp. Bot., July 1, 2006; 57(10): 2445 - 2453. [Abstract] [Full Text] [PDF]