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First published online February 17, 2006; 10.1104/pp.105.066993

Plant Physiology 140:1246-1254 (2006)
© 2006 American Society of Plant Biologists

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CELL BIOLOGY AND SIGNAL TRANSDUCTION

Chemical Imaging of Poplar Wood Cell Walls by Confocal Raman Microscopy

Notburga Gierlinger* and Manfred Schwanninger

Max-Planck-Institute of Colloids and Interfaces, Department of Biomaterials, 14424 Potsdam, Germany (N.G.); and BOKU-University of Natural Resources and Applied Life Sciences, Department of Chemistry, 1190 Vienna, Austria (M.S.)

Confocal Raman microscopy was used to illustrate changes of molecular composition in secondary plant cell wall tissues of poplar (Populus nigra x Populus deltoids) wood. Two-dimensional spectral maps were acquired and chemical images calculated by integrating the intensity of characteristic spectral bands. This enabled direct visualization of the spatial variation of the lignin content without any chemical treatment or staining of the cell wall. A small (0.5 µm) lignified border toward the lumen was observed in the gelatinous layer of poplar tension wood. The variable orientation of the cellulose was also characterized, leading to visualization of the S1 layer with dimensions smaller than 0.5 µm. Scanning Raman microscopy was thus shown to be a powerful, nondestructive tool for imaging changes in molecular cell wall organization with high spatial resolution.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Notburga Gierlinger (gierlinger{at}mpikg.mpg.de).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.066993.

* Corresponding author; e-mail gierlinger{at}mpikg.mpg.de; fax 49–331–567–9402.

Received June 14, 2005; returned for revision January 12, 2006; accepted January 29, 2006.


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