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First published online February 17, 2006; 10.1104/pp.105.074138 Plant Physiology 140:1345-1354 (2006) © 2006 American Society of Plant Biologists Light-Regulated, Tissue-Specific, and Cell Differentiation-Specific Expression of the Arabidopsis Fe(III)-Chelate Reductase Gene AtFRO61State Key Laboratory of Plant Genomics (H.F., F.A., S.Z., Z.J., J.Z.) and State Key Laboratory of Plant Cell and Chromosome Engineering (H.-Q.L.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China, 100101; and Graduate School, Chinese Academy of Sciences, Beijing, China, 100049 (H.F., F.A., S.Z., Z.J.)
Iron is an essential element for almost all living organisms, actively involved in a variety of cellular activities. To acquire iron from soil, strategy I plants such as Arabidopsis (Arabidopsis thaliana) must first reduce ferric to ferrous iron by Fe(III)-chelate reductases (FROs). FRO genes display distinctive expression patterns in several plant species. However, regulation of FRO genes is not well understood. Here, we report a systematic characterization of the AtFRO6 expression during plant growth and development. AtFRO6, encoding a putative FRO, is specifically expressed in green-aerial tissues in a light-dependent manner. Analysis of mutant promoter-
1 This work was supported by the National Natural Science Foundation (grant nos. 30125025 and 30221002 to J.Z.; grant nos. 30225029 and 30530460 to H.-Q.L.), by the Chinese Academy of Sciences (grant no. KSCX2–SW–308 to J.Z.), and by HarvestPlus Program-China (to H.-Q.L.). 2 These authors contributed equally to the paper. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Jianru Zuo (jrzuo{at}genetics.ac.cn). Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.074138. * Corresponding author; e-mail jrzuo{at}genetics.ac.cn; fax 8610–6487–3428. Received November 15, 2005; returned for revision January 26, 2006; accepted February 6, 2006. This article has been cited by other articles:
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-glucuronidase reporter genes in transgenic Arabidopsis plants revealed the presence of multiple light-responsive elements in the AtFRO6 promoter. These light-responsive elements may act synergistically to confer light responsiveness to the AtFRO6 promoter. Moreover, no AtFRO6 expression was detected in dedifferentiated green calli of the korrigan1-2 (kor1-2) mutant or undifferentiated calli derived from wild-type explants. Conversely, AtFRO6 is expressed in redifferentiated kor1-2 shoot-like structures and differentiating calli of wild-type explants. In addition, AtFRO7, but not AtFRO5 and AtFRO8, also shows a reduced expression level in kor1-2 green calli. These results suggest that whereas photosynthesis is necessary but not sufficient, both light and cell differentiation are necessary for AtFRO6 expression. We propose that AtFRO6 expression is light regulated in a tissue- or cell differentiation-specific manner to facilitate the acquisition of iron in response to distinctive developmental cues. 
