This Article Full Text Full Text (PDF) Supplemental Data Supplemental Data All Versions of this Article: 140/4/1355    most recent pp.106.077990v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Sozzani, R. Articles by Cella, R. Search for Related Content PubMed PubMed Citation Articles by Sozzani, R. Articles by Cella, R. Agricola Articles by Sozzani, R. Articles by Cella, R.

SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION

Interplay between Arabidopsis Activating Factors E2Fb and E2Fa in Cell Cycle Progression and Development^1,[W]

Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy (R.S., C.M., R.C.); Department of Environmental Agronomy and Crop Production, University of Padova, Agripolis, 35020 Legnaro, Italy (S.V., S.C.); Institute of Plant Biotechnology, University of Paris-XI, 91400 Orsay, France (C.B.); and Department of Botany and Plant Ecology, University of Sassari, 07100 Sassari, Italy (D.A.)

Eukaryotic E2Fs are conserved transcription factors playing crucial and antagonistic roles in several pathways related to cell division, DNA repair, and differentiation. In plants, these processes are strictly intermingled at the growing zone to produce postembryonic development in response to internal signals and environmental cues. Of the six AtE2F proteins found in Arabidopsis (Arabidopsis thaliana), only AtE2Fa and AtE2Fb have been clearly indicated as activators of E2F-responsive genes. AtE2Fa activity was shown to induce S phase and endoreduplication, whereas the function of AtE2Fb and the interrelationship between these two transcription factors was unclear. We have investigated the role played by the AtE2Fb gene during cell cycle and development performing in situ RNA hybridization, immunolocalization of the AtE2Fb protein in planta, and analysis of AtE2Fb promoter activity in transgenic plants. Overexpression of AtE2Fb in transgenic Arabidopsis plants led to striking modifications of the morphology of roots, cotyledons, and leaves that can be ascribed to stimulation of cell division. The accumulation of the AtE2Fb protein in these lines was paralleled by an increased expression of E2F-responsive G1/S and G2/M marker genes. These results suggest that AtE2Fa and AtE2Fb have specific expression patterns and play similar but distinct roles during cell cycle progression.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Rino Cella (cella{at}ipvgen.unipv.it).

^[W] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.077990.

^* Corresponding author; e-mail cella{at}ipvgen.unipv.it; fax 39–0382–528496.

Received January 27, 2006; returned for revision February 13, 2006; accepted February 14, 2006.

This article has been cited by other articles:

				V. Boudolf, T. Lammens, J. Boruc, J. Van Leene, H. Van Den Daele, S. Maes, G. Van Isterdael, E. Russinova, E. Kondorosi, E. Witters, et al. CDKB1;1 Forms a Functional Complex with CYCA2;3 to Suppress Endocycle Onset Plant Physiology, July 1, 2009; 150(3): 1482 - 1493. [Abstract] [Full Text] [PDF]

				K. Kato, I. Galis, S. Suzuki, S. Araki, T. Demura, M.-C. Criqui, T. Potuschak, P. Genschik, H. Fukuda, K. Matsuoka, et al. Preferential Up-Regulation of G2/M Phase-Specific Genes by Overexpression of the Hyperactive Form of NtmybA2 Lacking Its Negative Regulation Domain in Tobacco BY-2 Cells Plant Physiology, April 1, 2009; 149(4): 1945 - 1957. [Abstract] [Full Text] [PDF]

				R. C. Day, R. P. Herridge, B. A. Ambrose, and R. C. Macknight Transcriptome Analysis of Proliferating Arabidopsis Endosperm Reveals Biological Implications for the Control of Syncytial Division, Cytokinin Signaling, and Gene Expression Regulation Plant Physiology, December 1, 2008; 148(4): 1964 - 1984. [Abstract] [Full Text] [PDF]

				J. T. Ascencio-Ibanez, R. Sozzani, T.-J. Lee, T.-M. Chu, R. D. Wolfinger, R. Cella, and L. Hanley-Bowdoin Global Analysis of Arabidopsis Gene Expression Uncovers a Complex Array of Changes Impacting Pathogen Response and Cell Cycle during Geminivirus Infection Plant Physiology, September 1, 2008; 148(1): 436 - 454. [Abstract] [Full Text] [PDF]

				E. Ramirez-Parra and C. Gutierrez E2F Regulates FASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status Plant Physiology, May 1, 2007; 144(1): 105 - 120. [Abstract] [Full Text] [PDF]

				J. C. del Pozo, S. Diaz-Trivino, N. Cisneros, and C. Gutierrez The Balance between Cell Division and Endoreplication Depends on E2FC-DPB, Transcription Factors Regulated by the Ubiquitin-SCFSKP2A Pathway in Arabidopsis PLANT CELL, September 1, 2006; 18(9): 2224 - 2235. [Abstract] [Full Text] [PDF]