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First published online February 24, 2006; 10.1104/pp.105.074690

Plant Physiology 140:1484-1493 (2006)
© 2006 American Society of Plant Biologists

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PLANTS INTERACTING WITH OTHER ORGANISMS

Adducts of Oxylipin Electrophiles to Glutathione Reflect a 13 Specificity of the Downstream Lipoxygenase Pathway in the Tobacco Hypersensitive Response

Céline Davoine1, Olivier Falletti, Thierry Douki, Gilles Iacazio, Najla Ennar, Jean-Luc Montillet and Christian Triantaphylidès*

Laboratoire de Radiobiologie Végétale, DSV-Département d'Ecophysiologie Végétale et de Microbiologie, CEA-Cadarache, 13108 Saint-Paul Lez Durance cedex, France (C.D., N.E., J.-L.M., C.T.); Laboratoire Lésions des Acides Nucléiques, Service de Chimie Inorganique et Biologique, DSM-Département de Recherche Fondamentale sur la Matière Condensée, Unité Mixte de Recherche-E 3 CEA-UJF, CEA-Grenoble, 38054 Grenoble cedex 9, France (O.F., T.D.); and Laboratoire de Bioinorganique Structurale, Case 432, Unité Mixte de Recherche Centre National de la Recherche Scientifique 6517 Chimie, Biologie et Radicaux libres, Faculté des Sciences de Saint Jérôme, 13397 Marseille cedex 20, France (G.I.)

The response to reactive electrophile species (RES) is now considered as part of the plant response to pathogen and insect attacks. Thanks to a previously established high-performance liquid chromatography tandem mass spectrometry methodology, we have investigated the production of oxylipin RES adducts to glutathione (GSH) during the hypersensitive response (HR) of plants. We have observed that RES conjugation to GSH in tobacco (Nicotiana tabacum) leaves is facile and nonspecific. In cryptogein-elicited tobacco leaves, we show that the oxylipin RES adducts to GSH are produced in correlation with GSH consumption, increase in glutathione S-transferase activity, and the appearance of the cell death symptoms. In this model, the adducts arise mainly from the downstream 13 lipoxygenase (LOX) metabolism, although the induced 9 LOX pathway leads massively to the accumulation of upstream metabolites. The main adducts were obtained from 2-hexenal and 12-oxo-phytodienoic acid. They accumulate transiently as 1-hexanol-3-GSH, a reduced adduct, and 12-oxo-phytodienoic acid-GSH, respectively. RES conjugation does not initiate cell death but explains part of the GSH depletion that accompanies HR cell death. The nature of these GSH conjugates shows the key role played by the 13 LOX pathway in RES signaling in the tobacco HR.

1 Present address: Gene Expression Laboratory, Plant Molecular Biology, University of Lausanne, Biophore Building, 1015 Lausanne, Switzerland.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Christian Triantaphylidès (ctriantaphylid{at}cea.fr).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.074690.

* Corresponding author; e-mail ctriantaphylid{at}cea.fr; fax 33–4–42–25–26–25.

Received November 24, 2005; returned for revision February 8, 2006; accepted February 10, 2006.




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