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First published online March 31, 2006; 10.1104/pp.106.079038

Plant Physiology 141:243-256 (2006)
© 2006 American Society of Plant Biologists

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DEVELOPMENT AND HORMONE ACTION

G-Protein Complex Mutants Are Hypersensitive to Abscisic Acid Regulation of Germination and Postgermination Development1,[W]

Sona Pandey2, Jin-Gui Chen2,3, Alan M. Jones and Sarah M. Assmann*

Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802–5301 (S.P., S.M.A.); and Departments of Biology and Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599–3280 (J.-G.C., A.M.J.)

Abscisic acid (ABA) plays regulatory roles in a host of physiological processes throughout plant growth and development. Seed germination, early seedling development, stomatal guard cell functions, and acclimation to adverse environmental conditions are key processes regulated by ABA. Recent evidence suggests that signaling processes in both seeds and guard cells involve heterotrimeric G proteins. To assess new roles for the Arabidopsis (Arabidopsis thaliana) G{alpha} subunit (GPA1), the Gbeta subunit (AGB1), and the candidate G-protein-coupled receptor (GCR1) in ABA signaling during germination and early seedling development, we utilized knockout mutants lacking one or more of these components. Our data show that GPA1, AGB1, and GCR1 each negatively regulates ABA signaling in seed germination and early seedling development. Plants lacking AGB1 have greater ABA hypersensitivity than plants lacking GPA1, suggesting that AGB1 is the predominant regulator of ABA signaling and that GPA1 affects the efficacy of AGB1 execution. GCR1 acts upstream of GPA1 and AGB1 for ABA signaling pathways during germination and early seedling development: gcr1 gpa1 double mutants exhibit a gpa1 phenotype and agb1 gcr1 and agb1 gcr1 gpa1 mutants exhibit an agb1 phenotype. Contrary to the scenario in guard cells, where GCR1 and GPA1 have opposite effects on ABA signaling during stomatal opening, GCR1 acts in concert with GPA1 and AGB1 in ABA signaling during germination and early seedling development. Thus, cell- and tissue-specific functional interaction in response to a given signal such as ABA may determine the distinct pathways regulated by the individual members of the G-protein complex.

1 This work was supported by the National Science Foundation (grant no. MCB–0209694 to S.M.A. and grant no. MCB–0209711 to A.M.J.) and the National Institute of General Medical Sciences (grant no. GM65989–01 to A.M.J.).

2 These authors contributed equally to the paper.

3 Present address: Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Sarah M. Assmann (sma3{at}psu.edu).

[W] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.079038.

* Corresponding author; e-mail sma3{at}psu.edu; fax 814–865–9131.

Received February 10, 2006; returned for revision March 17, 2006; accepted March 19, 2006.


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