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GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Gene Expression Profiling Using cDNA Microarray Analysis of the Sexual Reproduction Stage of the Unicellular Charophycean Alga Closterium peracerosum-strigosum-littorale Complex^1,[W]

Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112–8681, Japan (H. Sekimoto); Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, Tokyo 153–8902, Japan (Y. Tanabe, Y. Tsuchikane, H. Shirosaki, M.I.); Plant Science Center, Institute of Physical and Chemical Research, Yokohama 230–0045, Japan (H.F., T.D.); and Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113–0033, Japan (H.F.)

The desmid Closterium peracerosum-strigosum-littorale complex, which is the closest unicellular sister to land plants, is the best characterized of the charophycean green algae with respect to the process of sexual reproduction. To elucidate the molecular mechanism of intercellular communication during sexual reproduction, we created a normalized cDNA library from mixed cells of the sexual and the vegetative phases and generated a cDNA microarray. In total, 3,236 expressed sequence tags, which were classified into 1,615 nonredundant groups, were generated for cDNA microarray construction. Candidate genes for key factors involved in fertilization, such as those that encode putative receptor-like protein kinase, leucine-rich-repeat receptor-like protein, and sex pheromone homologs, were up-regulated during sexual reproduction and/or by the addition of the purified sex pheromones, and the expression patterns of these genes were confirmed by quantitative real-time polymerase chain reaction analysis. This first transcriptome profile of Closterium will provide critical clues as to the mechanism and evolution of intercellular communication between the egg and sperm cells of land plants.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Hiroyuki Sekimoto (sekimoto{at}fc.jwu.ac.jp).

^[W] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.078048.

^* Corresponding author; e-mail sekimoto{at}fc.jwu.ac.jp; fax 81–3–5981–3674.

Received January 28, 2006; returned for revision March 9, 2006; accepted March 11, 2006.

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