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BREAKTHROUGH TECHNOLOGIES

LucTrap Vectors Are Tools to Generate Luciferase Fusions for the Quantification of Transcript and Protein Abundance in Vivo¹

Centre for Plant Molecular Biology, Developmental Genetics, 72076 Tuebingen, Germany (L.I.A.C.-V., C.K., H.L., C.S.); GABI-Kat, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (M.R., B.W.); and Institute for Genome Research, Centre for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany (M.R., B.W.)

Proper plant growth and development strongly rely on the plant's ability to respond dynamically to signals and cues from the intra- and extracellular environment. Whereas many of these responses require specific changes at the level of gene expression, in recent years it has become increasingly clear that many plant responses are at least in part also controlled at the level of protein turnover. It is a challenge for signal transduction research to understand how distinct incoming signals are integrated to generate specific changes at the transcript or protein level. The activity of luciferase (LUC) reporters can be detected in nondestructive qualitative and quantitative assays in vivo. Therefore,z LUC reporters are particularly well suited for the detection of changes at the transcript and protein level. To the best of our knowledge, the number of plant transformation vectors for LUC fusions is very limited. In this article, we describe the LucTrap plant transformation vectors that allow generation of targeted and random transcriptional and translational fusions with the modified firefly LUC reporter LUC+. We demonstrate that LucTrap-based fusions can be used to monitor rapid changes in gene expression and protein abundance in vivo.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Claus Schwechheimer (claus.schwechheimer{at}zmbp.uni-tuebingen.de).

www.plantphysiol.org/cgi/doi/10.1104/pp.106.078097.

^* Corresponding author; e-mail claus.schwechheimer{at}zmbp.uni-tuebingen.de; fax 49–7071–295135.

Received January 30, 2006; returned for revision March 15, 2006; accepted March 15, 2006.

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