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Chloroplastic NAD(P)H Dehydrogenase in Tobacco Leaves Functions in Alleviation of Oxidative Damage Caused by Temperature Stress^1,[OA]

National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (P.W., J.-Y.Y., H.M.); Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (W.D.); Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Sakyo, Kyoto 606–8502, Japan (A.T., T.E.); and Graduate School of Agriculture, Kyushu University, Hakozaki, Higashiku, Fukuoka 812–8581, Japan (T.S.)

In this study, the function of the NAD(P)H dehydrogenase (NDH)-dependent pathway in suppressing the accumulation of reactive oxygen species in chloroplasts was investigated. Hydrogen peroxide accumulated in the leaves of tobacco (Nicotiana tabacum) defective in ndhC-ndhK-ndhJ (ndhCKJ) at 42°C and 4°C, and in that of wild-type leaves at 4°C. The maximum quantum efficiency of PSII decreased to a similar extent in both strains at 42°C, while it decreased more evidently in ndhCKJ at 4°C. The parameters linked to CO₂ assimilation, such as the photochemical efficiency of PSII, the decrease of nonphotochemical quenching following the initial rise, and the photosynthetic O₂ evolution, were inhibited more significantly in ndhCKJ than in wild type at 42°C and were seriously inhibited in both strains at 4°C. While cyclic electron flow around PSI mediated by NDH was remarkably enhanced at 42°C and suppressed at 4°C. The proton gradient across the thylakoid membranes and light-dependent ATP synthesis were higher in wild type than in ndhCKJ at either 25°C or 42°C, but were barely formed at 4°C. Based on these results, we suggest that cyclic photophosphorylation via the NDH pathway might play an important role in regulation of CO₂ assimilation under heat-stressed condition but is less important under chilling-stressed condition, thus optimizing the photosynthetic electron transport and reducing the generation of reactive oxygen species.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Hualing Mi (mihl{at}iris.sipp.ac.cn).

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Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.070490.

^* Corresponding author; e-mail mihl{at}iris.sipp.ac.cn; fax 86–21–54924015.

Received August 28, 2005; returned for revision October 21, 2005; accepted October 25, 2005.

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