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Mitogen-Activated Protein Kinase Is Involved in Abscisic Acid-Induced Antioxidant Defense and Acts Downstream of Reactive Oxygen Species Production in Leaves of Maize Plants¹

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China (A.Z., M.J., M.T., X.H.); and Department of Biology, Hong Kong Baptist University, Hong Kong, People's Republic of China (J.Z.)

The role of mitogen-activated protein kinase (MAPK) in abscisic acid (ABA)-induced antioxidant defense was investigated in leaves of maize (Zea mays) plants. Treatments with ABA or H₂O₂ induced the activation of a 46-kD MAPK and enhanced the expression of the antioxidant genes CAT1, cAPX, and GR1 and the total activities of the antioxidant enzymes catalase, ascorbate peroxidase, glutathione reductase, and superoxide dismutase. Such enhancements were blocked by pretreatment with several MAPK kinase inhibitors and reactive oxygen species inhibitors or scavengers. Pretreatment with MAPK kinase inhibitors also substantially arrested the ABA-induced H₂O₂ production after 2 h of ABA treatment, but did not affect the levels of H₂O₂ within 1 h of ABA treatment. Pretreatment with several inhibitors of protein tyrosine phosphatase, which is believed to be a negative regulator of MAPK, only slightly prevented the ABA-induced H₂O₂ production, but did not affect the ABA-induced MAPK activation and ABA-enhanced antioxidant defense systems. These results clearly suggest that MAPK but not protein tyrosine phosphatase is involved in the ABA-induced antioxidant defense, and a cross talk between H₂O₂ production and MAPK activation plays a pivotal role in the ABA signaling. ABA-induced H₂O₂ production activates MAPK, which in turn induces the expression and the activities of antioxidant enzymes. The activation of MAPK also enhances the H₂O₂ production, forming a positive feedback loop.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Mingyi Jiang (myjiang{at}njau.edu.cn).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.075416.

^* Corresponding author; e-mail myjiang{at}njau.edu.cn; fax 86–25–84396673.

Received December 11, 2005; returned for revision January 12, 2006; accepted January 13, 2006.

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