First published online April 14, 2006; 10.1104/pp.106.080523
Plant Physiology 141:587-597 (2006)
© 2006 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
Subcellular Localization and Membrane Topology of the Melon Ethylene Receptor CmERS11
Biao Ma,
Min-Long Cui,
Hyeon-Jin Sun,
Keita Takada,
Hitoshi Mori,
Hiroshi Kamada and
Hiroshi Ezura*
Gene Research Center, University of Tsukuba, Tsukuba, Ibaraki 305–8572, Japan (B.M., M.-L.C., H.-J.S., K.T., H.K., H.E.); and Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464–8601, Japan (H.M.)
Ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. To better understand their biochemical functions, we investigated the membrane topology and subcellular localization of CmERS1, a melon (Cucumis melo) ethylene receptor that has three putative transmembrane domains at the N terminus. Analyses using membrane fractionation and green fluorescent protein imaging approaches indicate that CmERS1 is predominantly associated with the endoplasmic reticulum (ER) membrane. Detergent treatments of melon microsomes showed that the receptor protein is integrally bound to the ER membrane. A protease protection assay and N-glycosylation analysis were used to determine membrane topology. The results indicate that CmERS1 spans the membrane three times, with its N terminus facing the luminal space and the large C-terminal portion lying on the cytosolic side of the ER membrane. This orientation provides a platform for interaction with the cytosolic signaling elements. The three N-terminal transmembrane segments were found to function as topogenic sequences to determine the final topology. High conservation of these topogenic sequences in all ethylene receptor homologs identified thus far suggests that these proteins may share the same membrane topology.
1 This work was supported by a grant-in-aid from the Research for the Future program of the Japan Society for the Promotion of Science (grant no. JSPS–RFTF00L01601) and by a grant from the 21st Century Centers of Excellence Program of the Ministry of Education, Culture, Sports, Science and Technology, and in part by funds from the National Institute for Basic Biology Cooperative Research Program (grant no. 5–138 to H.E.).
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Hiroshi Ezura (ezura{at}gene.tsukuba.ac.jp).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.080523.
* Corresponding author; e-mail ezura{at}gene.tsukuba.ac.jp; fax 81–29–853–6006.
Received March 15, 2006;
returned for revision April 9, 2006;
accepted April 9, 2006.
This article has been cited by other articles:
|
|
|
|
 
Z. Lin, C.-W. Ho, and D. Grierson
AtTRP1 encodes a novel TPR protein that interacts with the ethylene receptor ERS1 and modulates development in Arabidopsis
J. Exp. Bot.,
September 1, 2009;
60(13):
3697 - 3714.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chen, J. Liu, G. Lei, Y.-F. Liu, Z.-G. Li, J.-J. Tao, Y.-J. Hao, Y.-R. Cao, Q. Lin, W.-K. Zhang, et al.
Effects of Tobacco Ethylene Receptor Mutations on Receptor Kinase Activity, Plant Growth and Stress Responses
Plant Cell Physiol.,
September 1, 2009;
50(9):
1636 - 1650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Lin, S. Zhong, and D. Grierson
Recent advances in ethylene research
J. Exp. Bot.,
August 1, 2009;
60(12):
3311 - 3336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Wuriyanghan, B. Zhang, W.-H. Cao, B. Ma, G. Lei, Y.-F. Liu, W. Wei, H.-J. Wu, L.-J. Chen, H.-W. Chen, et al.
The Ethylene Receptor ETR2 Delays Floral Transition and Affects Starch Accumulation in Rice
PLANT CELL,
May 1, 2009;
21(5):
1473 - 1494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Lin, L. Arciga-Reyes, S. Zhong, L. Alexander, R. Hackett, I. Wilson, and D. Grierson
SlTPR1, a tomato tetratricopeptide repeat protein, interacts with the ethylene receptors NR and LeETR1, modulating ethylene and auxin responses and development
J. Exp. Bot.,
November 1, 2008;
59(15):
4271 - 4287.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Grefen, K. Stadele, K. Ruzicka, P. Obrdlik, K. Harter, and J. Horak
Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members
Mol Plant,
March 1, 2008;
1(2):
308 - 320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zhong, Z. Lin, and D. Grierson
Tomato ethylene receptor-CTR interactions: visualization of NEVER-RIPE interactions with multiple CTRs at the endoplasmic reticulum
J. Exp. Bot.,
March 1, 2008;
59(4):
965 - 972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zhou, Q. Liu, F. Xie, and C.-K. Wen
RTE1 Is a Golgi-Associated and ETR1-Dependent Negative Regulator of Ethylene Responses
Plant Physiology,
September 1, 2007;
145(1):
75 - 86.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
