First published online April 28, 2006; 10.1104/pp.106.080267
Plant Physiology 141:737-744 (2006)
© 2006 American Society of Plant Biologists
BIOENERGETICS AND PHOTOSYNTHESIS
CHLORORESPIRATORY REDUCTION 6 Is a Novel Factor Required for Accumulation of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis1
M. Kamruzzaman Munshi,
Yoshichika Kobayashi and
Toshiharu Shikanai*
Graduate School of Agriculture, Kyushu University, Fukuoka 812–8581, Japan
The chloroplast NAD(P)H dehydrogenase (NDH) complex is involved in photosystem I cyclic electron transport and chlororespiration in higher plants. An Arabidopsis (Arabidopsis thaliana) chlororespiratory reduction 6 (crr6) mutant lacking NDH activity was identified by means of chlorophyll fluorescence imaging. Accumulation of the NDH complex was impaired in crr6. Physiological characterization of photosynthetic electron transport indicated the specific defect of the NDH complex in crr6. In contrast to the CRR7 protein that was recently identified as a potential novel subunit of the NDH complex by means of the same screening, the CRR6 protein was stable under the crr2 mutant background in which the NDH complex does not accumulate. The CRR6 gene (At2g47910) encodes a novel protein without any known motif. Although CRR6 does not have any transmembrane domains, it is localized in the thylakoid membrane fraction of the chloroplast. CRR6 is conserved in phototrophs, including cyanobacteria, from which the chloroplast NDH complex has evolutionally originated, but not in Chlamydomonas reinhardtii, in which the NDH complex is absent. We believe that CRR6 is a novel specific factor for the assembly or stabilization of the NDH complex.
1 This work was supported by a grant-in-aid for Scientific Research on Priority Areas (grant no. 16085206) and for Creative Scientific Research (grant no. 17GS0316) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Toshiharu Shikanai (shikanai{at}agr.kyushu-u.ac.jp).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.080267.
* Corresponding author; email shikanai{at}agr.kyushu-u.ac.jp; fax 092–642–2882.
Received March 14, 2006;
returned for revision April 14, 2006;
accepted April 18, 2006.
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