First published online April 7, 2006; 10.1104/pp.106.079293
Plant Physiology 141:776-792 (2006)
© 2006 American Society of Plant Biologists
GENETICS, GENOMICS, AND MOLECULAR EVOLUTION
Transcription Analysis of Arabidopsis Membrane Transporters and Hormone Pathways during Developmental and Induced Leaf Senescence1,[W]
Eric van der Graaff2,
Rainer Schwacke,
Anja Schneider3,
Marcelo Desimone,
Ulf-Ingo Flügge and
Reinhard Kunze4,*
Institute of Botany II, University of Cologne, 50931 Cologne, Germany (E.v.d.G., R.S., A.S., U.-I.F., R.K.); and Center of Plant Molecular Biology, University of Tübingen, 72076 Tuebingen, Germany (M.D.)
A comparative transcriptome analysis for successive stages of Arabidopsis (Arabidopsis thaliana) developmental leaf senescence (NS), darkening-induced senescence of individual leaves attached to the plant (DIS), and senescence in dark-incubated detached leaves (DET) revealed many novel senescence-associated genes with distinct expression profiles. The three senescence processes share a high number of regulated genes, although the overall number of regulated genes during DIS and DET is about 2 times lower than during NS. Consequently, the number of NS-specific genes is much higher than the number of DIS- or DET-specific genes. The expression profiles of transporters (TPs), receptor-like kinases, autophagy genes, and hormone pathways were analyzed in detail. The Arabidopsis TPs and other integral membrane proteins were systematically reclassified based on the Transporter Classification system. Coordinate activation or inactivation of several genes is observed in some TP families in all three or only in individual senescence types, indicating differences in the genetic programs for remobilization of catabolites. Characteristic senescence type-specific differences were also apparent in the expression profiles of (putative) signaling kinases. For eight hormones, the expression of biosynthesis, metabolism, signaling, and (partially) response genes was investigated. In most pathways, novel senescence-associated genes were identified. The expression profiles of hormone homeostasis and signaling genes reveal additional players in the senescence regulatory network.
1 This work was supported by grants from the Deutsche Forschungsgemeinschaft, the Bundesministerium für Bildung und Forschung, and the Fonds der Chemischen Industrie.
2 Present address: Institute of Biology III, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
3 Present address: Botanical Institute, Ludwig-Maximilians-University Munich, Menzinger Str. 67, 80638 Munich, Germany.
4 Present address: Institute of Biology/Applied Genetics, Free University Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin, Germany.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Reinhard Kunze (rkunze{at}zedat.fu-berlin.de).
[W] The online version of this article contains Web-only data.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.079293.
* Corresponding author; e-mail rkunze{at}zedat.fu-berlin.de; fax 49–30–838–54345.
Received February 15, 2006;
returned for revision April 4, 2006;
accepted April 5, 2006.
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