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SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION

Desensitization of GSTF8 Induction by a Prior Chemical Treatment Is Long Lasting and Operates in a Tissue-Dependent Manner^1,[W]

Commonwealth Scientific and Industrial Research Organization Plant Industry, Centre for Environmental and Life Sciences, Wembley, Western Australia 6913, Australia (R.C.F., P.G.S., R.P.-T., K.B.S.); and Australian Research Council Centre of Excellence in Plant Energy Biology and School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley, Western Australia 6009, Australia (P.G.S., A.H.M.)

The Arabidopsis (Arabidopsis thaliana) GSTF8 gene is a member of the glutathione S-transferase (GST) family whose expression is induced by defense signals, certain chemical stresses, and some pathogens. Here, we have used transgenic plants and an in vivo imaging system to demonstrate that GSTF8 expression is subject to a distinct desensitization phenomenon because prior chemical treatment significantly reduces reactivation of the GSTF8 promoter by hydrogen peroxide, auxin, and salicylic acid. A GSTF8 null line had similar desensitization properties to wild type, demonstrating that GSTF8 protein levels are not responsible for desensitization. The resulting refractory period is unusually long lasting, with full recovery taking 4 d. Expression of the GSTF8 promoter following a second treatment occurred predominantly in newly formed tissue at the root tip, suggesting that desensitization is lost upon cell division. Expression of the endogenous GSTF8 gene and another GST gene, GSTF6, is also desensitized following treatment with hydrogen peroxide. The desensitization phenomenon can be activated by a very low concentration of inducer that is not sufficient to activate the GSTF8 promoter. These results demonstrate that activation of the GSTF8 promoter is not essential for eliciting desensitization. A key promoter sequence within the GSTF8 gene, the ocs element, is also affected by desensitization. Treatment with a phosphatase inhibitor prevents desensitization of GSTF8 expression and ocs element activity, suggesting that dephosphorylation of one or more proteins is required for desensitization to occur.

² Present address: Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Karam B. Singh (karam.singh{at}csiro.au).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.079509

^* Corresponding author; e-mail karam.singh{at}csiro.au; fax 61–8–9387–8991.

Received February 21, 2006; accepted June 26, 2006.

This article has been cited by other articles:

				L. F. Thatcher, C. Carrie, C. R. Andersson, K. Sivasithamparam, J. Whelan, and K. B. Singh Differential Gene Expression and Subcellular Targeting of Arabidopsis Glutathione S-Transferase F8 Is Achieved through Alternative Transcription Start Sites J. Biol. Chem., September 28, 2007; 282(39): 28915 - 28928. [Abstract] [Full Text] [PDF]