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ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Hormonal and Stress Induction of the Gene Encoding Common Bean Acetyl-Coenzyme A Carboxylase^1,[W]

Plant Molecular Biology, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, Mexico

Regulation of the cytosolic acetyl-coenzyme A carboxylase (ACCase) gene promoter from common bean (Phaseolus vulgaris) was studied in transgenic Arabidopsis (Arabidopsis thaliana) plants using a -glucuronidase (GUS) reporter gene fusion (PvACCase::GUS). Under normal growth conditions, GUS was expressed in hydathodes, stipules, trichome bases, flowers, pollen, and embryos. In roots, expression was observed in the tip, elongation zone, hypocotyl-root transition zone, and lateral root primordia. The PvACCase promoter was induced by wounding, Pseudomonas syringae infection, hydrogen peroxide, jasmonic acid (JA), ethylene, or auxin treatment. Analysis of PvACCase::GUS expression in JA and ethylene mutants (coronatine insensitive1-1 [coi1-1], ethylene resistant1-1 [etr1-1], coi1-1/etr1-1) suggests that neither JA nor ethylene perception participates in the activation of this gene in response to wounding, although each of these independent signaling pathways is sufficient for pathogen or hydrogen peroxide-induced PvACCase gene expression. We propose a model involving different pathways of PvACCase gene activation in response to stress.

² Present address: Functional Ecology, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad Universitaria c.p. 04510, México City, D.F., México.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Mario Rocha-Sosa (rocha{at}ibt.unam.mx).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.085597

^* Corresponding author; e-mail rocha{at}ibt.unam.mx; fax 52–777–3172388.

Received June 23, 2006; accepted August 18, 2006; published August 25, 2006.