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GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Self-Compatibility of Two Apricot Selections Is Associated with Two Pollen-Part Mutations of Different Nature^1,[W]

Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada Valencia, Spain (S.V., M.L.B., J.M-C., G.L., C.R.); and Departamento de Mejora de Frutales, CEBAS-CSIC, 30100 Murcia, Spain (L.B.)

Loss of pollen-S function in Prunus self-compatible mutants has recently been associated with deletions or insertions in S-haplotype-specific F-box (SFB) genes. We have studied two self-compatible cultivars of apricot (Prunus armeniaca), Currot (S_CS_C) and Canino (S₂S_C), sharing the naturally occurring self-compatible (S_C)-haplotype. Sequence analysis showed that whereas the S_C-RNase is unaltered, a 358-bp insertion is found in the SFB_C gene, resulting in the expression of a truncated protein. The alteration of this gene is associated with self-incompatibility (SI) breakdown, supporting previous evidence that points to SFB being the pollen-S gene of the Prunus SI S-locus. On the other hand, PCR analysis of progenies derived from Canino showed that pollen grains carrying the S₂-haplotype were also able to overcome the incompatibility barrier. However, alterations in the SFB₂ gene or evidence of pollen-S duplications were not detected. A new class of F-box genes encoding a previously uncharacterized protein with high sequence similarity (approximately 62%) to Prunus SFB proteins was identified in this work, but the available data rules them out of producing S-heteroallelic pollen and thus the cause of the pollen-part mutation. These results suggest that cv Canino has an additional mutation, not linked to the S-locus, which causes a loss of pollen-S activity when present in pollen. As a whole, these findings support the proposal that the S-locus products besides other S-locus independent factors are required for gametophytic SI in Prunus.

² Present address: Departamento de Genética, IRTA Centre de Cabrils, Ctra. de Cabrils, s/n. 08348 Cabrils Barcelona, Spain.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Carlos Romero (cromero{at}ivia.es).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.083865

^* Corresponding author; e-mail cromero{at}ivia.es; fax (34)–96–3424001.

Received May 19, 2006; accepted August 4, 2006; published August 18, 2006.

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