Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online August 4, 2006; 10.1104/pp.106.085647

Plant Physiology 142:750-761 (2006)
© 2006 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
142/2/750    most recent
pp.106.085647v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, M.
Right arrow Articles by Wang, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, M.
Right arrow Articles by Wang, X.
Agricola
Right arrow Articles by Li, M.
Right arrow Articles by Wang, X.
ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Quantitative Profiling of Arabidopsis Polar Glycerolipids in Response to Phosphorus Starvation. Roles of Phospholipases D{zeta}1 and D{zeta}2 in Phosphatidylcholine Hydrolysis and Digalactosyldiacylglycerol Accumulation in Phosphorus-Starved Plants1,[W]

Maoyin Li, Ruth Welti and Xuemin Wang*

Department of Biology, University of Missouri, St. Louis, Missouri 63121 (M.L., X.W.); Danforth Plant Science Center, St. Louis, Missouri 63132 (M.L., X.W.); and Division of Biology, Kansas State University, Manhattan, Kansas 66506 (R.W.)

Phosphorus is an essential macronutrient that often limits plant growth and development. Under phosphorus-limited conditions, plants undergo substantial alterations in membrane lipid composition to cope with phosphorus deficiency. To characterize the changes in lipid species and to identify enzymes involved in plant response to phosphorus starvation, 140 molecular species of polar glycerolipids were quantitatively profiled in rosettes and roots of wild-type Arabidopsis (Arabidopsis thaliana) and phospholipase D knockout mutants pld{zeta}1, pld{zeta}2, and pld{zeta}1pld{zeta}2. In response to phosphorus starvation, the concentration of phospholipids was decreased and that of galactolipids was increased. Phospholipid lost in phosphorus-starved Arabidopsis rosettes was replaced by an equal amount of galactolipid. The concentration of phospholipid lost in roots was much greater than in rosettes. Disruption of both PLD{zeta}1 and PLD{zeta}2 function resulted in a smaller decrease in phosphatidylcholine and a smaller increase in digalactosyldiacylglycerol in phosphorus-starved roots. The results suggest that hydrolysis of phosphatidylcholine by PLD{zeta}s during phosphorus starvation contributes to the supply of inorganic phosphorus for cell metabolism and diacylglycerol moieties for galactolipid synthesis.

1 This work was supported by the National Science Foundation (NSF; grant nos. MCB–0455318 and IOB–0454866) and by the U.S. Department of Agriculture (grant no. 2005–35818–15253). The Kansas Lipidomics Research Center Analytical Laboratory was supported by the NSF EPSCoR program (grant no. EPS–0236913) with matching support from the State of Kansas through the Kansas Technology Enterprise Corporation and Kansas State University. The Kansas Lipidomics Research Center also received Core Facility support from the K-IDeA Networks of Biomedical Research Excellence (INBRE) through the National Institutes of Health (grant no. P20RR16475 from the INBRE program of the National Center for Research Resources). This is contribution 06–323–J from the Kansas Agricultural Experiment Station.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instruction for Authors (www.plantphysiol.org) is: Xuemin Wang (wangxue{at}umsl.edu).

[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.085647

* Corresponding author; e-mail wangxue{at}umsl.edu; fax 314–587–1519.

Received June 22, 2006; accepted July 28, 2006; published August 4, 2006.




This article has been cited by other articles:


Home page
 J Exp BotHome page
J. P. Hammond and P. J. White
Sucrose transport in the phloem: integrating root responses to phosphorus starvation
J. Exp. Bot., January 1, 2008; 59(1): 93 - 109.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists