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First published online September 29, 2006; 10.1104/pp.106.087056

Plant Physiology 142:1053-1064 (2006)
© 2006 American Society of Plant Biologists

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DEVELOPMENT AND HORMONE ACTION

The Cyclin-Dependent Kinase Inhibitor Orysa;KRP1 Plays an Important Role in Seed Development of Rice1,[W]

Rosa Maria Barrôco2, Adrian Peres2,3, Anne-Marie Droual2, Lieven De Veylder, Le Son Long Nguyen, Joris De Wolf, Vladimir Mironov, Rindert Peerbolte, Gerrit T.S. Beemster, Dirk Inzé*, Willem F. Broekaert and Valerie Frankard

Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B–9052 Ghent, Belgium (R.M.B., L.D.V., L.S.L.N., V.M., G.T.S.B., D.I.); and CropDesign NV, B–9052 Ghent, Belgium (A.P., A.-M.D., J.D.W., R.P., W.F.B., V.F.)

Kip-related proteins (KRPs) play a major role in the regulation of the plant cell cycle. We report the identification of five putative rice (Oryza sativa) proteins that share characteristic motifs with previously described plant KRPs. To investigate the function of KRPs in rice development, we generated transgenic plants overexpressing the Orysa;KRP1 gene. Phenotypic analysis revealed that overexpressed KRP1 reduced cell production during leaf development. The reduced cell production in the leaf meristem was partly compensated by an increased cell size, demonstrating the existence of a compensatory mechanism in monocot species by which growth rate is less reduced than cell production, through cell expansion. Furthermore, Orysa;KRP1 overexpression dramatically reduced seed filling. Sectioning through the overexpressed KRP1 seeds showed that KRP overproduction disturbed the production of endosperm cells. The decrease in the number of fully formed seeds was accompanied by a drop in the endoreduplication of endosperm cells, pointing toward a role of KRP1 in connecting endocycle with endosperm development. Also, spatial and temporal transcript detection in developing seeds suggests that Orysa;KRP1 plays an important role in the exit from the mitotic cell cycle during rice grain formation.

1 This work was supported in part by a grant from the Institute for the Promotion of Innovation by Science and Technology in Flanders (TraitQuest grant no. 000391 and postdoctoral fellowship to R.M.B.), the European Research Training Network (DAGOLIGN project HPRN–CT–2002–00267 [fellowship to A.P.] and Marie Curie Industry Host Fellowship Horyzan project HPMI–CT–1999–00056 [fellowship to A.-M.D.]), and the Research Foundation-Flanders (postdoctoral fellowship to L.D.V.).

2 These authors contributed equally to the paper.

3 Present address: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, B–2440 Geel, Belgium.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Valerie Frankard (valerie.frankard{at}cropdesign.com).

[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.087056

* Corresponding author; e-mail dirk.inze{at}psb.ugent.be; fax 32–9–3313809.

Received July 20, 2006; accepted September 21, 2006; published September 29, 2006.




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