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WHOLE PLANT AND ECOPHYSIOLOGY

Genetic Variation Suggests Interaction between Cold Acclimation and Metabolic Regulation of Leaf Senescence^1,[W],[OA]

Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, F–78026 Versailles cedex, France (C.M.-D., T.L.); Department of Biology, University College London, London WC1E 6BT, United Kingdom (S.P., N.P., A.W.); and Unité de Recherche en Génomique Végétale/Institut National de la Recherche Agronomique/Centre National de la Recherche Scientifique, Université d'Evry Val d'Essonne, F–91057 Evry cedex, France (L.T., J.-P.R.)

The extent to which leaf senescence is induced by nitrogen deficiency or by sugar accumulation varies between natural accessions of Arabidopsis (Arabidopsis thaliana). Analysis of senescence in plants of the Bay-0 x Shahdara recombinant inbred line (RIL) population revealed a large variation in developmental senescence of the whole leaf rosette, which was in agreement with the extent to which glucose (Glc) induced senescence in the different lines. To determine the regulatory basis of genetic differences in the Glc response, we investigated changes in gene expression using Complete Arabidopsis Transcriptome MicroArray (CATMA) analysis. Genes whose regulation did not depend on the genetic background, as well as genes whose regulation was specific to individual RILs, were identified. In RIL 310, a line that does not show the typical senescence response to Glc, stress response genes, especially those responding to cold stress, were induced by Glc. We therefore tested whether cold acclimation delays senescence by reducing sugar sensitivity. In cold-acclimated plants, leaf senescence was severely delayed and Glc did not induce the typical senescence response. Together, our results suggest that cold acclimation extends rosette longevity by affecting metabolic regulation of senescence, thereby allowing vernalization-dependent plants to survive the winter period. The role of functional chloroplasts and of nitrogen and phosphate availability in this regulation is discussed.

² Present address: Unité Mixte de Recherches 6553, Centre National de la Recherche Scientifique, Ecobio, Université de Rennes 1, Campus Scientifique de Beaulieu, Bat. 14A, F–35042 Rennes cedex, France.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Astrid Wingler (a.wingler{at}ucl.ac.uk).

^[W] The online version of this article contains Web-only data.

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www.plantphysiol.org/cgi/doi/10.1104/pp.106.091355

^* Corresponding author; e-mail a.wingler{at}ucl.ac.uk; fax 44–20–7679–7096.

Received October 17, 2006; accepted November 7, 2006; published November 10, 2006.

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