Plant Physiol.
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First published online December 1, 2006; 10.1104/pp.106.090126

Plant Physiology 143:570-578 (2007)
© 2007 American Society of Plant Biologists

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BREAKTHROUGH TECHNOLOGIES

Transposition-Based Plant Transformation

Hua Yan and Caius M. Rommens*

Simplot Plant Sciences, J.R. Simplot Company, Boise, Idaho 83706

Agrobacterium T-DNAs were used to deliver transposable Dissociation (Ds) elements into the nuclei of potato (Solanum tuberosum) cells. A double-selection system was applied to enrich for plants that only contained a transposed Ds element. This system consisted of a positive selection for the neomycin phosphotransferase (nptII) gene positioned within Ds followed by a negative selection against stable integration of the cytosine deaminase (codA) gene-containing T-DNA. Sixteen of 29 transgenic plants were found to contain a transposed element while lacking any superfluous T-DNA sequences. The occurrence of this genotype indicates that Ds elements can transpose from relatively short extrachromosomal DNA molecules into the plant genome. The frequency of single-copy Ds transformation was determined at 0.3%, which is only about 2.5-fold lower than the potato transformation frequency for backbone-free and single-copy T-DNAs. Because of the generally high expression levels of genes positioned within transposed elements, the new transformation method may find broad applicability to crops that are accessible to Agrobacterium T-DNA transfer.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Caius M. Rommens (crommens{at}simplot.com).

www.plantphysiol.org/cgi/doi/10.1104/pp.106.090126

* Corresponding author; e-mail crommens{at}simplot.com; fax 208–327–3212.

Received September 20, 2006; accepted November 23, 2006; published December 1, 2006.

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