OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 143/2/784    most recent pp.106.090837v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Little, D. Articles by Reymond, P. Search for Related Content PubMed PubMed Citation Articles by Little, D. Articles by Reymond, P. Agricola Articles by Little, D. Articles by Reymond, P. Related Collections Plant-Herbivore Interactions

PLANTS INTERACTING WITH OTHER ORGANISMS

Oviposition by Pierid Butterflies Triggers Defense Responses in Arabidopsis^1,[W],[OA]

Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland

Insect eggs represent a threat for the plant as hatching larvae rapidly start with their feeding activity. Using a whole-genome microarray, we studied the expression profile of Arabidopsis (Arabidopsis thaliana) leaves after oviposition by two pierid butterflies. For Pieris brassicae, the deposition of egg batches changed the expression of hundreds of genes over a period of 3 d after oviposition. The transcript signature was similar to that observed during a hypersensitive response or in lesion-mimic mutants, including the induction of defense and stress-related genes and the repression of genes involved in growth and photosynthesis. Deposition of single eggs by Pieris rapae caused a similar although much weaker transcriptional response. Analysis of the jasmonic acid and salicylic acid mutants coi1-1 and sid2-1 indicated that the response to egg deposition is mostly independent of these signaling pathways. Histochemical analyses showed that egg deposition is causing a localized cell death, accompanied by the accumulation of callose, and the production of reactive oxygen species. In addition, activation of the pathogenesis-related1::-glucuronidase reporter gene correlated precisely with the site of egg deposition and was also triggered by crude egg extract. This study provides molecular evidence for the detection of egg deposition by Arabidopsis plants and suggests that oviposition causes a localized response with strong similarity to a hypersensitive response.

The author responsible for distribution of materials integral to the findings presented in this article in accord with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Philippe Reymond (philippe.reymond{at}unil.ch).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.090837

^* Corresponding author; e-mail philippe.reymond{at}unil.ch; fax 0041–21–692–4195.

Received October 4, 2006; accepted November 21, 2006; published December 1, 2006.

This article has been cited by other articles:

				J. P. Klingler, R. M. Nair, O. R. Edwards, and K. B. Singh A single gene, AIN, in Medicago truncatula mediates a hypersensitive response to both bluegreen aphid and pea aphid, but confers resistance only to bluegreen aphid J. Exp. Bot., October 1, 2009; 60(14): 4115 - 4127. [Abstract] [Full Text] [PDF]

				C. Cosio and C. Dunand Specific functions of individual class III peroxidase genes J. Exp. Bot., February 1, 2009; 60(2): 391 - 408. [Abstract] [Full Text] [PDF]

				N. E. Fatouros, C. Broekgaarden, G. Bukovinszkine'Kiss, J. J. A. van Loon, R. Mumm, M. E. Huigens, M. Dicke, and M. Hilker From the Cover: Male-derived butterfly anti-aphrodisiac mediates induced indirect plant defense PNAS, July 22, 2008; 105(29): 10033 - 10038. [Abstract] [Full Text] [PDF]

				A. Koornneef and C. M.J. Pieterse Cross Talk in Defense Signaling Plant Physiology, March 1, 2008; 146(3): 839 - 844. [Full Text] [PDF]

				K. Zhu-Salzman, D. S. Luthe, and G. W. Felton Arthropod-Inducible Proteins: Broad Spectrum Defenses against Multiple Herbivores Plant Physiology, March 1, 2008; 146(3): 852 - 858. [Full Text] [PDF]

				M. de Vos, K. L. Kriksunov, and G. Jander Indole-3-Acetonitrile Production from Indole Glucosinolates Deters Oviposition by Pieris rapae Plant Physiology, March 1, 2008; 146(3): 916 - 926. [Abstract] [Full Text] [PDF]

				N. E. Fatouros, M. Dicke, R. Mumm, T. Meiners, and M. Hilker Foraging behavior of egg parasitoids exploiting chemical information Behav. Ecol., February 27, 2008; (2008) arn011v1. [Abstract] [Full Text] [PDF]

				H. Abe, J. Ohnishi, M. Narusaka, S. Seo, Y. Narusaka, S. Tsuda, and M. Kobayashi Function of Jasmonate in Response and Tolerance of Arabidopsis to Thrip Feeding Plant Cell Physiol., January 1, 2008; 49(1): 68 - 80. [Abstract] [Full Text] [PDF]

				G. Bonaventure, A. Gfeller, V. M. Rodriguez, F. Armand, and E. E. Farmer The fou2 Gain-of-Function Allele and the Wild-Type Allele of Two Pore Channel 1 Contribute to Different Extents or by Different Mechanisms to Defense Gene Expression in Arabidopsis Plant Cell Physiol., December 1, 2007; 48(12): 1775 - 1789. [Abstract] [Full Text] [PDF]

				H. Yang, S. Yang, Y. Li, and J. Hua The Arabidopsis BAP1 and BAP2 Genes Are General Inhibitors of Programmed Cell Death Plant Physiology, September 1, 2007; 145(1): 135 - 146. [Abstract] [Full Text] [PDF]