This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 143/2/893    most recent pp.106.092270v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by March-Díaz, R. Articles by Reyes, J. C. Search for Related Content PubMed PubMed Citation Articles by March-Díaz, R. Articles by Reyes, J. C. Agricola Articles by March-Díaz, R. Articles by Reyes, J. C.

SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION

SEF, a New Protein Required for Flowering Repression in Arabidopsis, Interacts with PIE1 and ARP6^1,[W]

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, E–41092 Seville, Spain (R.M.D., M.G.D., F.J.F., J.C.R.); and Centro Andaluz de Biología Molecular y Medicina Regenerativa, E–41092 Seville, Spain (M.G.D., J.C.R.)

The SWR1/SRCAP complex is a chromatin-remodeling complex that has been shown to be involved in substitution of histone H2A by the histone variant H2A.Z in yeast (Saccharomyces cerevisiae) and animals. Here, we identify and characterize SERRATED LEAVES AND EARLY FLOWERING (SEF), an Arabidopsis (Arabidopsis thaliana) homolog of the yeast SWC6 protein, a conserved subunit of the SWR1/SRCAP complex. SEF loss-of-function mutants present a pleiotropic phenotype characterized by serrated leaves, frequent absence of inflorescence internodes, bushy aspect, and flowers with altered number and size of organs. sef plants flower earlier than wild-type plants both under inductive and noninductive photoperiods. This correlates with strong reduction of FLOWERING LOCUS C and MADS-AFFECTING FLOWERING4 transcript levels and up-regulation of FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene expression. The sef phenotype is similar to that of the photoperiod-independent early flowering1 (pie1) and the actin-related protein 6 (arp6) mutants. PIE1 and ARP6 proteins are also homologs of SWR1/SRCAP complex subunits. Analysis of sef pie1 double mutants demonstrates genetic interaction between these two genes. We also show physical interactions between SEF, ARP6, and PIE1 proteins. Taken together, our data indicate that SEF, ARP6, and PIE1 might form a molecular complex in Arabidopsis related to the SWR1/SRCAP complex identified in other eukaryotes.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: José C. Reyes (jose.reyes{at}cabimer.es).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.092270

^* Corresponding author; e-mail jose.reyes{at}cabimer.es; fax 34–954–461–664.

Received October 30, 2006; accepted November 22, 2006; published December 1, 2006.

This article has been cited by other articles:

				R. March-Diaz and J. C. Reyes The Beauty of Being a Variant: H2A.Z and the SWR1 Complex in Plants Mol Plant, March 31, 2009; (2009) ssp019v1. [Abstract] [Full Text] [PDF]

				R. J. Schmitz, Y. Tamada, M. R. Doyle, X. Zhang, and R. M. Amasino Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis Plant Physiology, February 1, 2009; 149(2): 1196 - 1204. [Abstract] [Full Text] [PDF]

				B. Ben Amor, S. Wirth, F. Merchan, P. Laporte, Y. d'Aubenton-Carafa, J. Hirsch, A. Maizel, A. Mallory, A. Lucas, J. M. Deragon, et al. Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses Genome Res., January 1, 2009; 19(1): 57 - 69. [Abstract] [Full Text] [PDF]

				C. M. Alexandre and L. Hennig FLC or not FLC: the other side of vernalization J. Exp. Bot., April 4, 2008; (2008) ern070v1. [Abstract] [Full Text] [PDF]

				A. Lazaro, A. Gomez-Zambrano, L. Lopez-Gonzalez, M. Pineiro, and J. A. Jarillo Mutations in the Arabidopsis SWC6 gene, encoding a component of the SWR1 chromatin remodelling complex, accelerate flowering time and alter leaf and flower development J. Exp. Bot., February 21, 2008; (2008) erm332v1. [Abstract] [Full Text] [PDF]

				L. Xu, Z. Zhao, A. Dong, L. Soubigou-Taconnat, J.-P. Renou, A. Steinmetz, and W.-H. Shen Di- and Tri- but Not Monomethylation on Histone H3 Lysine 36 Marks Active Transcription of Genes Involved in Flowering Time Regulation and Other Processes in Arabidopsis thaliana Mol. Cell. Biol., February 15, 2008; 28(4): 1348 - 1360. [Abstract] [Full Text] [PDF]

				C. R. Andersson, C. A. Helliwell, D. J. Bagnall, T. P. Hughes, E. J. Finnegan, W. J. Peacock, and E. S. Dennis The FLX Gene of Arabidopsis is Required for FRI-Dependent Activation of FLC Expression Plant Cell Physiol., February 1, 2008; 49(2): 191 - 200. [Abstract] [Full Text] [PDF]