This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 143/2/912    most recent pp.106.090738v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Kleffmann, T. Articles by Baginsky, S. Search for Related Content PubMed PubMed Citation Articles by Kleffmann, T. Articles by Baginsky, S. Agricola Articles by Kleffmann, T. Articles by Baginsky, S.

SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION

Proteome Dynamics during Plastid Differentiation in Rice^1,[W]

Institute of Plant Sciences, Eidgenössische Technische Hochschule Zurich, 8092 Zurich, Switzerland (T.K., A.v.Z., D.R., M.H.-H., W.G., S.B.); and Functional Genomics Center Zurich, 8057 Zurich, Switzerland (P.G., W.G.)

We have analyzed proteome dynamics during light-induced development of rice (Oryza sativa) chloroplasts from etioplasts using quantitative two-dimensional gel electrophoresis and tandem mass spectrometry protein identification. In the dark, the etioplast allocates the main proportion of total protein mass to carbohydrate and amino acid metabolism and a surprisingly high number of proteins to the regulation and expression of plastid genes. Chaperones, proteins for photosynthetic energy metabolism, and enzymes of the tetrapyrrole pathway were identified among the most abundant etioplast proteins. The detection of 13 N-terminal acetylated peptides allowed us to map the exact localization of the transit peptide cleavage site, demonstrating good agreement with the prediction for most proteins. Based on the quantitative etioplast proteome map, we examined early light-induced changes during chloroplast development. The transition from heterotrophic metabolism to photosynthesis-supported autotrophic metabolism was already detectable 2 h after illumination and affected most essential metabolic modules. Enzymes in carbohydrate metabolism, photosynthesis, and gene expression were up-regulated, whereas enzymes in amino acid and fatty acid metabolism were significantly decreased in relative abundance. Enzymes involved in nucleotide metabolism, tetrapyrrole biosynthesis, and redox regulation remained unchanged. Phosphoprotein-specific staining at different time points during chloroplast development revealed light-induced phosphorylation of a nuclear-encoded plastid RNA-binding protein, consistent with changes in plastid RNA metabolism. Quantitative information about all identified proteins and their regulation by light is available in plprot, the plastid proteome database (http://www.plprot.ethz.ch).

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Sacha Baginsky (sbaginsky{at}ethz.ch).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.090738

^* Corresponding author; e-mail sbaginsky{at}ethz.ch; fax 41–1–632–10–79.

Received October 2, 2006; accepted December 7, 2006; published December 22, 2006.

This article has been cited by other articles:

				T. Aki, M. Shigyo, R. Nakano, T. Yoneyama, and S. Yanagisawa Nano Scale Proteomics Revealed the Presence of Regulatory Proteins Including Three FT-Like proteins in Phloem and Xylem Saps from Rice Plant Cell Physiol., May 1, 2008; 49(5): 767 - 790. [Abstract] [Full Text] [PDF]

				E. Kanervo, M. Singh, M. Suorsa, V. Paakkarinen, E. Aro, N. Battchikova, and E.-M. Aro Expression of Protein Complexes and Individual Proteins Upon Transition of Etioplasts to Chloroplasts in Pea (Pisum sativum) Plant Cell Physiol., March 1, 2008; 49(3): 396 - 410. [Abstract] [Full Text] [PDF]