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First published online January 26, 2007; 10.1104/pp.106.091546 Plant Physiology 143:1220-1230 (2007) © 2007 American Society of Plant Biologists OsCSLD1, a Cellulose Synthase-Like D1 Gene, Is Required for Root Hair Morphogenesis in Rice1,[C],[W]Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660–701, Korea (C.M.K., S.H.P., B.I.J., S.H.P., S.J.P., H.L.P., C.-d.H.); Rice Functional Genomics, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441–707, Korea (M.Y.E.); and Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (L.D.)
Root hairs are long tubular outgrowths that form on the surface of specialized epidermal cells. They are required for nutrient and water uptake and interact with the soil microflora. Here we show that the Oryza sativa cellulose synthase-like D1 (OsCSLD1) gene is required for root hair development, as rice (Oryza sativa) mutants that lack OsCSLD1 function develop abnormal root hairs. In these mutants, while hair development is initiated normally, the hairs elongate less than the wild-type hairs and they have kinks and swellings along their length. Because the csld1 mutants develop the same density and number of root hairs along their seminal root as the wild-type plants, we propose that OsCSLD1 function is required for hair elongation but not initiation. Both gene trap expression pattern and in situ hybridization analyses indicate that OsCSLD1 is expressed in only root hair cells. Furthermore, OsCSLD1 is the only member of the four rice CSLD genes that shows root-specific expression. Given that the Arabidopsis (Arabidopsis thaliana) gene KOJAK/AtCSLD3 is required for root hair elongation and is expressed in the root hair, it appears that OsCSLD1 may be the functional ortholog of KOJAK/AtCSLD3 and that these two genes represent the root hair-specific members of this family of proteins. Thus, at least part of the mechanism of root hair morphogenesis in Arabidopsis is conserved in rice.
1 This work was supported by the Crop Functional Genomics Center of the 21st Century Frontier Research Program, BioGreen 21 Program (Rural Development Administration; grant no. CG151), by the Korea Research Foundation (grant no. KRF–2003–015–C00636), by the Korea Science and Engineering Foundation/Ministry of Science and Technology to the Environmental Biotechnology National Core Research Center (grant no. R15–2003–012–01001–0), and by the Brain Korea21 project (fellowships to B.I.J., S.J.P., and H.L.P.). The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Chang-deok Han (cdhan{at}gsnu.ac.kr). [C] Some figures in this article are displayed in color online but in black and white in the print edition. [W] The online version of this article contains Web-only data. www.plantphysiol.org/cgi/doi/10.1104/pp.106.091546 * Corresponding author; e-mail cdhan{at}gsnu.ac.kr; fax 82–55–759–9363. Received October 19, 2006; accepted January 14, 2007; published January 26, 2007. This article has been cited by other articles:
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