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First published online January 5, 2007; 10.1104/pp.106.092817 Plant Physiology 143:1327-1346 (2007) © 2007 American Society of Plant Biologists OPEN ACCESS ARTICLE
Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening[OA]Institut National de la Recherche Agronomique, Unité de Génétique et Amélioration des Fruits et Légumes, INRA, UR 1052, Domaine Saint-Maurice, 84143 Montfavet cedex, France (M.F., C.M., M.C.); Institut National de la Recherche Agronomique, Unité Plantes et Systèmes de Culture Horticoles, INRA, UR 1115, Domaine Saint-Paul, 84914 Avignon cedex 9, France (N.B.); Instytut Dendrologii, Polska Akademia Nauk, 62–035 Kornik, Poland (T.P.); Institut National de la Recherche Agronomique, Unité Mixte de Recherche de Génétique Végétale, Institut Fédératif de Recherche 87, Plate-Forme de Protéomique du Moulon, 91190 Gif-sur-Yvette, France (L.N.); and Institut National de la Recherche Agronomique, Unité de Recherches Protéomique, INRA, UR 1199, 34000 Montpellier, France (N.S.)
Tomato (Solanum lycopersicum) is a model plant for studying fleshy fruit development. Several genetic and molecular approaches have been developed to increase our knowledge about the physiological basis of fruit growth, but very few data are yet available at the proteomic level. The main stages of fruit development were first determined through the dynamics of fruit diameter and pericarp cell number. Then, total proteins were extracted from pericarp tissue at six relevant developmental stages and separated by two-dimensional gel electrophoresis. Protein patterns were markedly different between stages. Proteins showing major variations were monitored. We identified 90 of 1,791 well-resolved spots either by matrix-assisted laser-desorption ionization time-of-flight peptide mass fingerprinting or liquid chromatography-mass spectrometry sequencing and expressed sequence tag database searching. Clustered correlation analysis results pointed out groups of proteins with similar expression profiles during fruit development. In young fruit, spots linked to amino acid metabolism or protein synthesis were mainly expressed during the cell division stage and down-regulated later. Some spots linked to cell division processes could be identified. During the cell expansion phase, spots linked to photosynthesis and proteins linked to cell wall formation transiently increased. In contrast, the major part of the spots related to C compounds and carbohydrate metabolism or oxidative processes were up-regulated during fruit development, showing an increase in spot intensity during development and maximal abundance in mature fruit. This was also the case for spots linked to stress responses and fruit senescence. We discuss protein variations, taking into account their potential role during fruit growth and comparing our results with already known variations at mRNA and metabolite-profiling levels.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Mireille Faurobert (mireille.faurobert{at}avignon.inra.fr). [OA] Open Access articles can be viewed online without a subscription. www.plantphysiol.org/cgi/doi/10.1104/pp.106.092817 * Corresponding author; e-mail mireille.faurobert{at}avignon.inra.fr; fax 33–4–32–72–27–02. Received November 9, 2006; accepted December 20, 2006; published January 5, 2007. This article has been cited by other articles:
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