First published online January 12, 2007; 10.1104/pp.106.091561
Plant Physiology 143:1362-1371 (2007)
© 2007 American Society of Plant Biologists
OPEN ACCESS ARTICLE
SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION
Auxin Biosynthesis by the YUCCA Genes in Rice1,[W],[OA]
Yuko Yamamoto,
Noriko Kamiya,
Yoichi Morinaka,
Makoto Matsuoka* and
Takashi Sazuka
Bioscience and Biotechnology Center, Nagoya University Chikusa, Nagoya Aichi, 4648601, Japan
Although indole-3-acetic acid (IAA), the predominant auxin in plants, plays a critical role in various plant growth and developmental processes, its biosynthesis and regulation have not been clearly elucidated. To investigate the molecular mechanisms of IAA synthesis in rice (Oryza sativa), we identified seven YUCCA-like genes (named OsYUCCA1-7) in the rice genome. Plants overexpressing OsYUCCA1 exhibited increased IAA levels and characteristic auxin overproduction phenotypes, whereas plants expressing antisense OsYUCCA1 cDNA displayed defects that are similar to those of rice auxin-insensitive mutants. OsYUCCA1 was expressed in almost all of the organs tested, but its expression was restricted to discrete areas, including the tips of leaves, roots, and vascular tissues, where it overlapped with expression of a -glucuronidase reporter gene controlled by the auxin-responsive DR5 promoter. These observations are consistent with an important role for the rice enzyme OsYUCCA1 in IAA biosynthesis via the tryptophan-dependent pathway.
1 This work was supported by the Center of Excellence and the Japan Rice Genome Project of the Ministry of Agriculture, Forestry and Fisheries (grant-in-aid).
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Makoto Matsuoka (makoto{at}agr.nagoya-u.ac.jp).
[W] The online version of this article contains Web-only data.
[OA] Open Access articles can be viewed online without a subscription.
www.plantphysiol.org/cgi/doi/10.1104/pp.106.091561
* Corresponding author; e-mail makoto{at}agr.nagoya-u.ac.jp; fax 81527895226.
Received October 20, 2006;
accepted January 3, 2007;
published January 12, 2007.
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