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CELL BIOLOGY AND SIGNAL TRANSDUCTION

Overexpression of Arabidopsis AGD7 Causes Relocation of Golgi-Localized Proteins to the Endoplasmic Reticulum and Inhibits Protein Trafficking in Plant Cells^1,[C],[OA]

Center for Plant Intracellular Trafficking, Division of Molecular and Life Sciences, and Department of Biology, Pohang University of Science and Technology, Pohang, 790–784, Korea (M.K.M., S.J.K., J.S., I.H.); and Molecular Biotechnology Program, the Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.M., L.J.)

ADP ribosylation factor (Arf) GTPase-activating proteins (GAPs) promote the hydrolysis of GTP bound to Arfs to GDP, which plays a pivotal role in regulating Arfs by converting the active GTP-bound forms of these proteins into their inactive GDP-bound forms. Here, we investigated the biological role of AGD7, an Arf GAP homolog, in Arabidopsis (Arabidopsis thaliana). We show that AGD7 bears a highly conserved N-terminal region and a unique C-terminal region, interacts with Arf1 both in vitro and in vivo, and stimulates Arf1 GTPase activity in a phosphatidic acid-dependent manner in vitro. In plant cells, AGD7 localized to the Golgi complex, where its overexpression was found to inhibit the Golgi localization of -subunit of coat proteins and promote the relocation of Golgi proteins into the endoplasmic reticulum in both protoplasts and transgenic plants. Furthermore, overexpression of AGD7 inhibited anterograde trafficking of proteins from the endoplasmic reticulum. We propose that AGD7 functions as a GAP for Arf1 in the Golgi complex and plays a critical role in protein trafficking by controlling Arf1 activity.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Inhwan Hwang (ihhwang{at}postech.ac.kr).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

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www.plantphysiol.org/cgi/doi/10.1104/pp.106.095091

^* Corresponding author; e-mail ihhwang{at}postech.ac.kr; fax 82–54–279–8159.

Received December 20, 2006; accepted February 6, 2007; published February 16, 2007.

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