This Article Full Text Full Text (PDF) All Versions of this Article: 143/4/1695    most recent pp.106.094052v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Maisch, J. Articles by Nick, P. Search for Related Content PubMed PubMed Citation Articles by Maisch, J. Articles by Nick, P. Agricola Articles by Maisch, J. Articles by Nick, P.

DEVELOPMENT AND HORMONE ACTION

Actin Is Involved in Auxin-Dependent Patterning¹

Institute of Botany 1, University of Karlsruhe, D–76128 Karlsruhe, Germany

Polar transport of auxin has been identified as a central element of pattern formation. The polarity of auxin transport is linked to the cycling of pin-formed proteins, a process that is related to actomyosin-dependent vesicle traffic. To get insight into the role of actin for auxin transport, we used patterned cell division to monitor the polarity of auxin fluxes. We show that cell division in the tobacco (Nicotiana tabacum L. cv Bright-Yellow 2) cell line is partially synchronized and that this synchrony can be perturbed by inhibition of auxin transport by 1-N-naphthylphthalamic acid. To address the role of actin in this synchrony, we induced a bundled configuration of actin by overexpressing mouse talin. The bundling of actin impairs the synchrony of cell division and increases the sensitivity to 1-N-naphthylphthalamic acid. Addition of the polarly transported auxins indole-3-acetic acid and 1-naphthyl acetic acid (but not 2,4-dichlorophenoxyacetic acid) restored both the normal organization of actin and the synchrony of cell division. This study suggests that auxin controls its own transport by changing the state of actin filaments.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Jan Maisch (jan.maisch{at}botanik1.uni-karlsruhe.de).

www.plantphysiol.org/cgi/doi/10.1104/pp.106.094052

^* Corresponding author; e-mail jan.maisch{at}botanik1.uni-karlsruhe.de; fax 49–721–608–4193.

Received December 3, 2006; accepted February 21, 2007; published March 2, 2007.

This article has been cited by other articles:

				F. Qiao, J. Petrasek, and P. Nick Light can rescue auxin-dependent synchrony of cell division in a tobacco cell line J. Exp. Bot., October 30, 2009; (2009) erp319v1. [Abstract] [Full Text] [PDF]

				P. Nick, M.-J. Han, and G. An Auxin Stimulates Its Own Transport by Shaping Actin Filaments Plant Physiology, September 1, 2009; 151(1): 155 - 167. [Abstract] [Full Text] [PDF]

				N. Frey, J. Klotz, and P. Nick Dynamic Bridges--A Calponin-Domain Kinesin From Rice Links Actin Filaments and Microtubules in Both Cycling and Non-Cycling Cells Plant Cell Physiol., August 1, 2009; 50(8): 1493 - 1506. [Abstract] [Full Text] [PDF]

				I. Hakman, H. Hallberg, and J. Palovaara The polar auxin transport inhibitor NPA impairs embryo morphology and increases the expression of an auxin efflux facilitator protein PIN during Picea abies somatic embryo development Tree Physiol, April 1, 2009; 29(4): 483 - 496. [Abstract] [Full Text] [PDF]

				M. Amenos, I. Corrales, C. Poschenrieder, P. Illes, F. Baluska, and J. Barcelo Different Effects of Aluminum on the Actin Cytoskeleton and Brefeldin A-Sensitive Vesicle Recycling in Root Apex Cells of Two Maize Varieties Differing in Root Elongation Rate and Aluminum Tolerance Plant Cell Physiol., March 1, 2009; 50(3): 528 - 540. [Abstract] [Full Text] [PDF]

				J. Maisch, J. Fiserova, L. Fischer, and P. Nick Tobacco Arp3 is localized to actin-nucleating sites in vivo J. Exp. Bot., February 1, 2009; 60(2): 603 - 614. [Abstract] [Full Text] [PDF]

				P. Dhonukshe, I. Grigoriev, R. Fischer, M. Tominaga, D. G. Robinson, J. Hasek, T. Paciorek, J. Petrasek, D. Seifertova, R. Tejos, et al. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes PNAS, March 18, 2008; 105(11): 4489 - 4494. [Abstract] [Full Text] [PDF]