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DEVELOPMENT AND HORMONE ACTION

The Rice YABBY1 Gene Is Involved in the Feedback Regulation of Gibberellin Metabolism^1,[C],[W]

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (M.D., Y.Z., Q.M., Y.H., Q.Z.); Institut de Biotechnologie des Plantes, Université Paris sud 11, Orsay F–91405, France (D.-X.Z.); and Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (P.H.)

Gibberellin (GA) biosynthesis is regulated by feedback control providing a mechanism for GA homeostasis in plants. However, regulatory elements involved in the feedback control are not known. In this report, we show that a rice (Oryza sativa) YABBY1 (YAB1) gene had a similar expression pattern as key rice GA biosynthetic genes GA3ox2 and GA20ox2. Overexpression of YAB1 in transgenic rice resulted in a semidwarf phenotype that could be fully rescued by applied GA. Quantification of the endogenous GA content revealed increases of GA₂₀ and decreases of GA₁ levels in the overexpression plants, in which the transcripts of the biosynthetic gene GA3ox2 were decreased. Cosuppression of YAB1 in transgenic plants induced expression of GA3ox2. The repression of GA3ox2 could be obtained upon treatment by dexamethasone of transgenic plants expressing a YAB1-glucocorticoid receptor fusion. Importantly, we show that YAB1 bound to a GA-responsive element within the GA3ox2 promoter. In addition, the expression of YAB1 was deregulated in GA biosynthesis and signaling mutants and could be either transiently induced by GA or repressed by a GA inhibitor. Finally, either overexpression or cosuppression of YAB1 impaired GA-mediated repression of GA3ox2. These data together suggest that YAB1 is involved in the feedback regulation of GA biosynthesis in rice.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Dao-Xiu Zhou (dao-xiu.zhou{at}u-psud.fr).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.096586

^* Corresponding author; e-mail dao-xiu.zhou{at}u-psud.fr; fax 33–169153424.

Received January 25, 2007; accepted March 8, 2007; published March 16, 2007.

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