Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online March 9, 2007; 10.1104/pp.106.090365

Plant Physiology 144:18-31 (2007)
© 2007 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
144/1/18    most recent
pp.106.090365v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mazier, M.
Right arrow Articles by Lucas, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mazier, M.
Right arrow Articles by Lucas, H.
Agricola
Right arrow Articles by Mazier, M.
Right arrow Articles by Lucas, H.
BREAKTHROUGH TECHNOLOGIES

Successful Gene Tagging in Lettuce Using the Tnt1 Retrotransposon from Tobacco

Marianne Mazier*, Emmanuel Botton, Fabrice Flamain, Jean-Paul Bouchet, Béatrice Courtial1, Marie-Christine Chupeau, Yves Chupeau, Brigitte Maisonneuve and Hélène Lucas

Unité de Génétique et d'Amélioration des Fruits et Légumes, UR1502, Institut National de la Recherche Agronomique (INRA), F–84143 Montfavet cedex, France (M.M., E.B., F.F., J.-P.B., B.M.); and Laboratoire de Biologie Cellulaire, UR501, INRA, F–78026 Versailles cedex, France (B.C., M.-C.C., Y.C., H.L.)

The tobacco (Nicotiana tabacum) element Tnt1 is one of the few identified active retrotransposons in plants. These elements possess unique properties that make them ideal genetic tools for gene tagging. Here, we demonstrate the feasibility of gene tagging using the retrotransposon Tnt1 in lettuce (Lactuca sativa), which is the largest genome tested for retrotransposon mutagenesis so far. Of 10 different transgenic bushes carrying a complete Tnt1 containing T-DNA, eight contained multiple transposed copies of Tnt1. The number of transposed copies of the element per plant was particularly high, the smallest number being 28. Tnt1 transposition in lettuce can be induced by a very simple in vitro culture protocol. Tnt1 insertions were stable in the progeny of the primary transformants and could be segregated genetically. Characterization of the sequences flanking some insertion sites revealed that Tnt1 often inserted into genes. The progeny of some primary transformants showed phenotypic alterations due to recessive mutations. One of these mutations was due to Tnt1 insertion in the gibberellin 3beta-hydroxylase gene. Taken together, these results indicate that Tnt1 is a powerful tool for insertion mutagenesis especially in plants with a large genome.

1 Present address: Laboratoire National de la Protection des Végétaux, Unité de Détection des OGM, 93 Rue de Curembourg, 45404 Fleury-les-Aubrais cedex, France.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Marianne Mazier (mazier{at}avignon.inra.fr).

www.plantphysiol.org/cgi/doi/10.1104/pp.106.090365

* Corresponding author; e-mail mazier{at}avignon.inra.fr; fax 33–4–32–72–27–02.

Received September 26, 2006; accepted January 30, 2007; published March 9, 2007.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists