First published online March 16, 2007; 10.1104/pp.106.093997
Plant Physiology 144:218-231 (2007)
© 2007 American Society of Plant Biologists
OPEN ACCESS ARTICLE
ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS
Transcript Profiling of the Anoxic Rice Coleoptile[W],[OA]
Rasika Lasanthi-Kudahettige1,
Leonardo Magneschi1,
Elena Loreti,
Silvia Gonzali,
Francesco Licausi,
Giacomo Novi,
Ottavio Beretta,
Federico Vitulli,
Amedeo Alpi and
Pierdomenico Perata*
Plant and Crop Physiology Laboratory, Scuola Superiore Sant'Anna, 56124 Pisa, Italy (R.L.-K., L.M., S.G., F.L, G.N., P.P.); Istituto di Biologia e Biotecnologia Agraria/Consiglio Nazionale delle Ricerche, 56100 Pisa, Italy (E.L.); Department of Crop Plant Biology, University of Pisa, 56124 Pisa, Italy (A.A.); and Genopolis, Department of Biotechnology and Bioscience, University of Milano-Bicocca, 20126 Milan, Italy (O.B., F.V.)
Rice (Oryza sativa) seeds can germinate in the complete absence of oxygen. Under anoxia, the rice coleoptile elongates, reaching a length greater than that of the aerobic one. In this article, we compared and investigated the transcriptome of rice coleoptiles grown under aerobic and anaerobic conditions. The results allow drawing a detailed picture of the modulation of the transcripts involved in anaerobic carbohydrate metabolism, suggesting up-regulation of the steps required to produce and metabolize pyruvate and its derivatives. Sugars appear to play a signaling role under anoxia, with several genes indirectly up-regulated by anoxia-driven sugar starvation. Analysis of the effects of anoxia on the expansin gene families revealed that EXPA7 and EXPB12 are likely to be involved in rice coleoptile elongation under anoxia. Genes coding for ethylene response factors and heat shock proteins are among the genes modulated by anoxia in both rice and Arabidopsis (Arabidopsis thaliana). Identification of anoxia-induced ethylene response factors is suggestive because genes belonging to this gene family play a crucial role in rice tolerance to submergence, a process closely related to, but independent from, the ability to germinate under anoxia. Genes coding for some enzymes requiring oxygen for their activity are dramatically down-regulated under anoxia, suggesting the existence of an energy-saving strategy in the regulation of gene expression.
1 These authors contributed equally to the article.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Pierdomenico Perata (p.perata{at}sssup.it).
[W] The online version of this article contains Web-only data.
[OA] Open Access articles can be viewed online without a subscription.
www.plantphysiol.org/cgi/doi/10.1104/pp.106.093997
* Corresponding author; e-mail p.perata{at}sssup.it; fax 39–0502216532.
Received December 1, 2006;
accepted March 6, 2007;
published March 16, 2007.
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