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BIOENERGETICS AND PHOTOSYNTHESIS

Effects of the Lack of Phosphatidylglycerol on the Donor Side of Photosystem II^1,[OA]

Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, Tokyo 153–8902, Japan (I.S., N.M., H.W.); and Institute of Materials Science, University of Tsukuba, Ibaraki 305–8573, Japan (S.O., M.K.)

Our previous studies with the pgsA mutant of the cyanobacterium Synechocystis sp. PCC6803 (hereafter termed pgsA mutant), which is defective for the biosynthesis of phosphatidylglycerol (PG), revealed an important role for PG in the electron acceptor side of photosystem II (PSII), especially in the electron transport between plastoquinones Q_A and Q_B. This study now shows that PG also plays an important role in the electron donor side of PSII, namely, the oxygen-evolving system. Analyses of purified PSII complexes indicated that PSII from PG-depleted pgsA mutant cells sustained only approximately 50% of the oxygen-evolving activity compared to wild-type cells. Dissociation of the extrinsic proteins PsbO, PsbV, and PsbU, which are required for stabilization of the manganese (Mn) cluster, followed by the release of a Mn atom, was observed in PSII of the PG-depleted mutant cells. The released PsbO rebound to PSII when PG was added back to the PG-depleted mutant cells, even when de novo protein synthesis was inhibited. Changes in photosynthetic activity of the PG-depleted pgsA mutant cells induced by heat treatment or dark incubation resembled those of psbO, psbV, and psbU mutant cells. These results suggest that PG plays an important role in binding extrinsic proteins required for sustaining a functional Mn cluster on the donor side of PSII.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Hajime Wada (hwada{at}bio.c.u-tokyo.ac.jp).

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www.plantphysiol.org/cgi/doi/10.1104/pp.107.098731

^* Corresponding author; e-mail hwada{at}bio.c.u-tokyo.ac.jp; fax 81–3–5454–6656.

Received March 1, 2007; accepted May 14, 2007; published May 18, 2007.

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