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CELL BIOLOGY AND SIGNAL TRANSDUCTION

Phosphatidylinositol 4-Kinase Activation Is an Early Response to Salicylic Acid in Arabidopsis Suspension Cells^1,[W]

Ondej Krinke^*, Eric Ruelland, Olga Valentová, Chantal Vergnolle, Jean-Pierre Renou, Ludivine Taconnat, Matyá Flemr, Lenka Burketová and Alain Zachowski

Université Pierre et Marie Curie-Paris 6 and Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7180, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, Ivry-sur-Seine F–94200, France (O.K., E.R., C.V., M.F., A.Z.); Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague 166 28, Czech Republic (O.K., O.V., M.F.); Unité Mixte de Recherche, Institut National de la Recherche Agronomique 1165, Centre National de la Recherche Scientifique 8114, Unité de Recherche en Génomique Végétale, Evry F–91057, France (J.-P.R., L.T.); and Institute of Experimental Botany, v.v.i., Academy of Sciences of the Czech Republic, Prague 165 02, Czech Republic (L.B.)

Salicylic acid (SA) has a central role in defense against pathogen attack. In addition, its role in such diverse processes as germination, flowering, senescence, and thermotolerance acquisition has been documented. However, little is known about the early signaling events triggered by SA. Using Arabidopsis (Arabidopsis thaliana) suspension cells as a model, it was possible to show by in vivo metabolic phospholipid labeling with ³³P_i that SA addition induced a rapid and early (in few minutes) decrease in a pool of phosphatidylinositol (PI). This decrease paralleled an increase in PI 4-phosphate and PI 4,5-bisphosphate. These changes could be inhibited by two different inhibitors of type III PI 4-kinases, phenylarsine oxide and 30 µM wortmannin; no inhibitory effect was seen with 1 µM wortmannin, a concentration inhibiting PI 3-kinases but not PI 4-kinases. We therefore undertook a study of the effects of wortmannin on SA-responsive transcriptomes. Using the Complete Arabidopsis Transcriptome MicroArray chip, we could identify 774 genes differentially expressed upon SA treatment. Strikingly, among these genes, the response to SA of 112 of them was inhibited by 30 µM wortmannin, but not by 1 µM wortmannin.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Ondej Krinke (ondrej.krinke{at}vscht.cz).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.100842

^* Corresponding author; e-mail ondrej.krinke{at}vscht.cz; fax 420–220–445–167.

Received April 10, 2007; accepted May 7, 2007; published May 11, 2007.

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