First published online April 27, 2007; 10.1104/pp.107.099903
Plant Physiology 144:1467-1480 (2007)
© 2007 American Society of Plant Biologists
OPEN ACCESS ARTICLE
PLANTS INTERACTING WITH OTHER ORGANISMS
The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis1,[W],[OA]
Steven Footitt2,
Daniela Dietrich,
Aaron Fait,
Alisdair R. Fernie,
Michael J. Holdsworth,
Alison Baker and
Frederica L. Theodoulou*
Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (S.F., D.D., F.L.T.); Division of Agricultural and Environmental Sciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom (D.D., M.J.H.); Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany (A.F., A.R.F.); and Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (A.B.)
COMATOSE (CTS) encodes a peroxisomal ATP-binding cassette transporter required not only for -oxidation of storage lipids during germination and establishment, but also for biosynthesis of jasmonic acid and conversion of indole butyric acid to indole acetic acid. cts mutants exhibited reduced fertilization, which was rescued by genetic complementation, but not by exogenous application of jasmonic acid or indole acetic acid. Reduced fertilization was also observed in thiolase (kat2-1) and peroxisomal acyl-Coenzyme A synthetase mutants (lacs6-1,lacs7-1), indicating a general role for -oxidation in fertility. Genetic analysis revealed reduced male transmission of cts alleles and both cts pollen germination and tube growth in vitro were impaired in the absence of an exogenous carbon source. Aniline blue staining of pollinated pistils demonstrated that pollen tube growth was affected only when both parents bore the cts mutation, indicating that expression of CTS in either male or female tissues was sufficient to support pollen tube growth in vivo. Accordingly, abundant peroxisomes were detected in a range of maternal tissues. Although -aminobutyric acid levels were reduced in flowers of cts mutants, they were unchanged in kat2-1, suggesting that alterations in -aminobutyric acid catabolism do not contribute to the reduced fertility phenotype through altered pollen tube targeting. Taken together, our data support an important role for -oxidation in fertility in Arabidopsis (Arabidopsis thaliana) and suggest that this pathway could play a role in the mobilization of lipids in both pollen and female tissues.
1 This work was supported by the Biology and Biotechnology Research Council (grant nos. P19770 to A.B. and P19769 to F.L.T. and M.H.), by the Biology and Biotechnology Research Council, UK (grant-aided support to Rothamsted Research), and by the Minerva foundation for metabolite analysis performed at the Max Planck Institute (grant to A.F.).
2 Present address: Warwick HRI, Warwick University, Wellesbourne, Warwick CV35 9EF, UK.
[W] The online version of this article contains Web-only data.
[OA] Open Access articles can be viewed online without a subscription.
www.plantphysiol.org/cgi/doi/10.1104/pp.107.099903
* Corresponding author; e-mail freddie.theodoulou{at}bbsrc.ac.uk; fax 441582763010.
Received March 23, 2007;
accepted April 17, 2007;
published April 27, 2007.
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