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PLANTS INTERACTING WITH OTHER ORGANISMS

The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis^1,[W],[OA]

Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (S.F., D.D., F.L.T.); Division of Agricultural and Environmental Sciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom (D.D., M.J.H.); Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany (A.F., A.R.F.); and Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (A.B.)

COMATOSE (CTS) encodes a peroxisomal ATP-binding cassette transporter required not only for -oxidation of storage lipids during germination and establishment, but also for biosynthesis of jasmonic acid and conversion of indole butyric acid to indole acetic acid. cts mutants exhibited reduced fertilization, which was rescued by genetic complementation, but not by exogenous application of jasmonic acid or indole acetic acid. Reduced fertilization was also observed in thiolase (kat2-1) and peroxisomal acyl-Coenzyme A synthetase mutants (lacs6-1,lacs7-1), indicating a general role for -oxidation in fertility. Genetic analysis revealed reduced male transmission of cts alleles and both cts pollen germination and tube growth in vitro were impaired in the absence of an exogenous carbon source. Aniline blue staining of pollinated pistils demonstrated that pollen tube growth was affected only when both parents bore the cts mutation, indicating that expression of CTS in either male or female tissues was sufficient to support pollen tube growth in vivo. Accordingly, abundant peroxisomes were detected in a range of maternal tissues. Although -aminobutyric acid levels were reduced in flowers of cts mutants, they were unchanged in kat2-1, suggesting that alterations in -aminobutyric acid catabolism do not contribute to the reduced fertility phenotype through altered pollen tube targeting. Taken together, our data support an important role for -oxidation in fertility in Arabidopsis (Arabidopsis thaliana) and suggest that this pathway could play a role in the mobilization of lipids in both pollen and female tissues.

² Present address: Warwick HRI, Warwick University, Wellesbourne, Warwick CV35 9EF, UK.

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www.plantphysiol.org/cgi/doi/10.1104/pp.107.099903

^* Corresponding author; e-mail freddie.theodoulou{at}bbsrc.ac.uk; fax 44–1582–763010.

Received March 23, 2007; accepted April 17, 2007; published April 27, 2007.