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First published online June 7, 2007; 10.1104/pp.107.100867

Plant Physiology 144:1733-1741 (2007)
© 2007 American Society of Plant Biologists

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BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

How a Plant Lectin Recognizes High Mannose Oligosaccharides1,[C],[OA]

Abel Garcia-Pino, Lieven Buts, Lode Wyns, Anne Imberty and Remy Loris*

Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B–1050 Brussel, Belgium (A.G.-P., L.B., L.W., R.L.); Department of Molecular and Cellular Interactions, VIB, Pleinlaan 2, B–1050 Brussel, Belgium (A.G.-P., L.B., L.W., R.L.); and Centre de Recherches sur les Macromolécules Végétales, Centre National de la Recherche Scientifique (affiliated with Joseph Fourier), BP53, 38041 Grenoble cedex 09, France (A.I.)

The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Man{alpha}(1–2)Man{alpha}(1–6)[Man{alpha}(1–3)]Man{alpha}(1–. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.

1 This work was supported by the Vlaams Interuniversitair Instituut voor Biotechnologie, the Onderzoeksraad of the Vrije Universiteit Brussel, and the Fonds voor Wetenschappelijk Onderzoek Vlaanderen (post-doc fellowship to L.B.).

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Remy Loris (reloris{at}vub.ac.be).

[C] Some figures in this article are displayed in color online but in black and white in the print edition.

[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.100867

* Corresponding author; e-mail reloris{at}vub.ac.be; fax 32–2–6291963.

Received April 13, 2007; accepted June 3, 2007; published June 7, 2007.

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