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ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Exclusion of Na⁺ via Sodium ATPase (PpENA1) Ensures Normal Growth of Physcomitrella patens under Moderate Salt Stress¹

Plant Biochemistry Laboratory, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK–1871 Frederiksberg C, Copenhagen, Denmark (C.L.,); and Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia (D.P.D., A.K.J., M.T.)

The bryophyte Physcomitrella patens is unlike any other plant identified to date in that it possesses a gene that encodes an ENA-type Na⁺-ATPase. To complement previous work in yeast (Saccharomyces cerevisiae), we determined the importance of having a Na⁺-ATPase in planta by conducting physiological analyses of PpENA1 in Physcomitrella. Expression studies showed that PpENA1 is up-regulated by NaCl and, to a lesser degree, by osmotic stress. Maximal induction is obtained after 8 h at 60 mM NaCl or above. No other abiotic stress tested led to significant increases in PpENA1 expression. In the gametophyte, strong expression was confined to the rhizoids, stem, and the basal part of the leaf. In the protonemata, expression was ubiquitous with a few filaments showing stronger expression. At 100 mM NaCl, wild-type plants were able to maintain a higher K⁺-to-Na⁺ ratio than the PpENA1 (ena1) knockout gene, but at higher NaCl concentrations no difference was observed. Although no difference in chlorophyll content was observed between ena1 and wild type at 100 mM NaCl, the impaired Na⁺ exclusion in ena1 plants led to an approximately 40% decrease in growth.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Christina Lunde (chlu{at}life.ku.dk).

www.plantphysiol.org/cgi/doi/10.1104/pp.106.094946

^* Corresponding author; e-mail chlu{at}life.ku.dk; fax 45–35–33–33–33.

Received December 20, 2006; accepted June 4, 2007; published June 7, 2007.

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