Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803

doi:10.1104/pp.107.103341

HOME

HELP

FEEDBACK

SUBSCRIPTIONS

Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803¹^,[W]

Marion Eisenhut, Eneas Aguirre von Wobeser, Ludwig Jonas, Hendrik Schubert, Bas W. Ibelings, Hermann Bauwe, Hans C.P. Matthijs and Martin Hagemann^*

Universität Rostock, Institut für Biowissenschaften, Abteilung Pflanzenphysiologie (M.E., H.B., M.H.) und Ökologie (H.S.), D–18059 Rostock, Germany; University of Amsterdam, Institute for Biodiversity and Ecosystem Dynamics, NL–1018WS Amsterdam, The Netherlands (E.A.v.W., H.C.P.M.); Universität Rostock, Institut für Pathologie, Elektronenmikroskopisches Zentrum, D–18055 Rostock, Germany (L.J.); Netherlands Institute of Ecology, Centre for Limnology, Department of Foodweb Studies, NL–3631AC Nieuwersluis, The Netherlands (B.W.I.); and Eawag, Swiss Federal Institute of Aquatic Sciences and Technology, Centre of Ecology, Evolution, and Biogeochemistry, CH–6047 Kastanienbaum, Switzerland (B.W.I.)

Concerted changes in the transcriptional pattern and physiologicaltraits that result from long-term (here defined as up to 24h) limitation of inorganic carbon (C_i) have been investigatedfor the cyanobacterium Synechocystis sp. strain PCC 6803. Resultsfrom reverse transcription-polymerase chain reaction and genome-wideDNA microarray analyses indicated stable up-regulation of genesfor inducible CO₂ and HCO₃^– uptake systems and of therfb cluster that encodes enzymes involved in outer cell wallpolysaccharide synthesis. Coordinated up-regulation of photosystemI genes was further found and supported by a higher photosystemI content and activity under low C_i (LC) conditions. Bacterial-typeglycerate pathway genes were induced by LC conditions, in contrastto the genes for the plant-like photorespiratory C2 cycle. Down-regulationwas observed for nitrate assimilation genes and surprisinglyalso for almost all carboxysomal proteins. However, for thelatter the observed elongation of the half-life time of thelarge subunit of Rubisco protein may render compensation. Mutantsdefective in glycolate turnover ( ${Delta}$ glcD and ${Delta}$ gcvT) showed sometranscriptional changes under high C_i conditions that are characteristicfor LC conditions in wild-type cells, like a modest down-regulationof carboxysomal genes. Properties under LC conditions were comparableto LC wild type, including the strong response of genes encodinginducible high-affinity C_i uptake systems. Electron microscopyrevealed a conspicuous increase in number of carboxysomes percell in mutant ${Delta}$ glcD already under high C_i conditions. Thesedata indicate that an increased level of photorespiratory intermediatesmay affect carboxysomal components but does not intervene withthe expression of majority of LC inducible genes.

¹ This work was supported by a scholarship from Consejo Nacionalpara la Ciencia y Tecnología (Mexico) and by a grantfrom the Earth and Life Sciences Foundation (to E.A.v.W.), whichis subsidized by the Netherlands Organization for ScientificResearch (grant to Dr. Jef Huisman, University of Amsterdam).Financial support for setting up the array facility and forpurchase of the arrays used in this study was provided by theBootsma fonds at the Royal Academy of Arts and Sciences (grantto B.W.I.). This work was also supported by a grant from theDeutsche Forschungsgemeinschaft (to M.H.).

The author responsible for distribution of materials integralto the findings presented in this article in accordance withthe policy described in the Instructions for Authors (www.plantphysiol.org)is: Martin Hagemann (martin.hagemann{at}uni-rostock.de).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.103341

^{^*} Corresponding author; e-mail martin.hagemann{at}uni-rostock.de; fax 49–0–3814986112.

Received June 4, 2007; accepted June 24, 2007; published June 28, 2007.

This article has been cited by other articles:

D. Dienst, U. Duhring, H.-J. Mollenkopf, J. Vogel, J. Golecki, W. R. Hess, and A. Wilde
The cyanobacterial homologue of the RNA chaperone Hfq is essential for motility of Synechocystis sp. PCC 6803
Microbiology, October 1, 2008; 154(10): 3134 - 3143.
[Abstract] [Full Text] [PDF]

S. Laurent, J. Jang, A. Janicki, C.-C. Zhang, and S. Bedu
Inactivation of spkD, encoding a Ser/Thr kinase, affects the pool of the TCA cycle metabolites in Synechocystis sp. strain PCC 6803
Microbiology, July 1, 2008; 154(7): 2161 - 2167.
[Abstract] [Full Text] [PDF]

Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 68031,[W]

Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803¹^,[W]