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ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

AtREV1, a Y-Family DNA Polymerase in Arabidopsis, Has Deoxynucleotidyl Transferase Activity in Vitro^1,[W]

Radiation-Applied Biology Division, Japan Atomic Energy Agency, Takasaki, Gunma 370–1292, Japan (S.T., A.N.S., A.T.); and Radioisotope Research Center, Osaka University, Suita, Osaka 565–0871, Japan (K.S.)

To clarify the functions of the Arabidopsis thaliana REV1 (AtREV1) protein, we expressed it in Escherichia coli and purified it to near homogeneity. The deoxynucleotidyl transferase activity of the recombinant AtREV1 was examined in vitro using a primer extension assay. The recombinant AtREV1 transferred one or two nucleotides to the primer end. It efficiently inserted dCMP regardless of the opposite base. AtREV1 also inserted a dCMP opposite an apurinic/apyrimidinic site, which is physiologically generated or induced by various DNA-damaging agents. In contrast, AtREV1 had no insertion activities against UV-inducible DNA lesions as reported in yeast or mammalian system. Although the substrate specificity of AtREV1 was rather narrow in the presence of magnesium ion, it widened in the presence of manganese ion. These results suggest that AtREV1 serves as a deoxycytidyl transferase in plant cells.

² These authors contributed equally to the article.

³ Present address: Plant Functional Genomics Research Team, Plant Functional Genomics Research Group, Plant Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1–7–22, Tsurumi, Yokohama, Kanagawa 230–0045, Japan.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Ayako N. Sakamoto (sakamoto.ayako{at}jaea.go.jp).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.101980

^* Corresponding author; e-mail sakamoto.ayako{at}jaea.go.jp.

Received May 7, 2007; accepted August 31, 2007; published September 7, 2007.