OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 145/3/575    most recent pp.107.106203v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Plant Physiol. Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dembinsky, D. Articles by Hochholdinger, F. Search for Related Content PubMed PubMed Citation Articles by Dembinsky, D. Articles by Hochholdinger, F. Agricola Articles by Dembinsky, D. Articles by Hochholdinger, F.

GENOME ANALYSIS

Transcriptomic and Proteomic Analyses of Pericycle Cells of the Maize Primary Root^1,[W],[OA]

Center for Plant Molecular Biology, Department of General Genetics, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany (D.D., K.W., M.S., Y.L., F.H.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (Y.F., B.B.); Department of Genetics, Development, and Cell Biology (L.A.B., P.S.S.), Bioinformatics and Computational Biology Graduate Program (L.A.B., P.S.S.), Department of Statistics (D.N.), and Center for Plant Genomics (P.S.S.), Iowa State University, Ames, Iowa 50011–3650; and Proteome Center Tuebingen, Interfaculty Institute for Cell Biology, University of Tuebingen, 72076 Tuebingen, Germany (T.L., C.F., J.M., A.N.)

Each plant cell type expresses a unique transcriptome and proteome at different stages of differentiation dependent on its developmental fate. This study compared gene expression and protein accumulation in cell-cycle-competent primary root pericycle cells of maize (Zea mays) prior to their first division and lateral root initiation. These are the only root cells that maintain the competence to divide after they leave the meristematic zone. Pericycle cells of the inbred line B73 were isolated via laser capture microdissection. Microarray experiments identified 32 genes preferentially expressed in pericycle versus all other root cells that have left the apical meristem; selective subtractive hybridization identified seven genes preferentially expressed in pericycle versus central cylinder cells of the same root region. Transcription and protein synthesis represented the most abundant functional categories among these pericycle-specific genes. Moreover, 701 expressed sequence tags (ESTs) were generated from pericycle and central cylinder cells. Among those, transcripts related to protein synthesis and cell fate were significantly enriched in pericycle versus nonpericycle cells. In addition, 77 EST clusters not previously identified in maize ESTs or genomic databases were identified. Finally, among the most abundant soluble pericycle proteins separated via two-dimensional electrophoresis, 20 proteins were identified via electrospray ionization-tandem mass spectrometry, thus defining a reference dataset of the maize pericycle proteome. Among those, two proteins were preferentially expressed in the pericycle. In summary, these pericycle-specific gene expression experiments define the distinct molecular events during the specification of cell-cycle-competent pericycle cells prior to their first division and demonstrate that pericycle specification and lateral root initiation might be controlled by a different set of genes.

² These authors contributed equally to the article.

³ Present address: KWS SAAT AG, Maize Breeding Department, 37555 Einbeck, Germany.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Frank Hochholdinger (frank.hochholdinger{at}zmbp.uni-tuebingen.de).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.106203

^* Corresponding author; e-mail frank.hochholdinger{at}zmbp.uni-tuebingen.de.

Received July 25, 2007; accepted August 22, 2007; published August 31, 2007.

Related articles in Plant Physiol.:

On the Inside

Peter V. Minorsky
Plant Physiol. 2007 145: 573-574. [Full Text]  

This article has been cited by other articles:

				S. Cai and C. C. Lashbrook Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis ZINC FINGER PROTEIN2 Plant Physiology, March 1, 2008; 146(3): 1305 - 1321. [Abstract] [Full Text] [PDF]