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Novel Plant Transformation Vectors Containing the Superpromoter^1,[OA]

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907–1392 (L.-Y.L., M.E.K., B.B., S.B.G.); and Center for Plant Transformation and Department of Agronomy, Plant Science Institute, Ames, Iowa 50011–1010 (B.R.F., K.W.)

We developed novel plasmids and T-DNA binary vectors that incorporate a modified and more useful form of the superpromoter. The superpromoter consists of a trimer of the octopine synthase transcriptional activating element affixed to the mannopine synthase2' (mas2') transcriptional activating element plus minimal promoter. We tested a superpromoter-β-glucuronidaseA fusion gene in stably transformed tobacco (Nicotiana tabacum) and maize (Zea mays) plants and in transiently transformed maize Black Mexican Sweet protoplasts. In both tobacco and maize, superpromoter activity was much greater in roots than in leaves. In tobacco, superpromoter activity was greater in mature leaves than in young leaves, whereas in maize activity differed little among the tested aerial portions of the plant. When compared with other commonly used promoters (cauliflower mosaic virus 35S, mas2', and maize ubiquitin), superpromoter activity was approximately equivalent to those of the other promoters in both maize Black Mexican Sweet suspension cells and in stably transformed maize plants. The addition of a maize ubiquitin intron downstream of the superpromoter did not enhance activity in stably transformed maize.

² These authors contributed equally to this article.

³ Present address: Voyager Pharmaceutical Corp., 8640 Colonnade Dr., Suite 501, Raleigh, NC 27615.

⁴ Present address: Missouri Botanical Garden, P.O. Box 299, St. Louis, MO 63166–0299.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Stanton B. Gelvin (gelvin{at}bilbo.bio.purdue.edu).

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.106633

^* Corresponding author; e-mail gelvin{at}bilbo.bio.purdue.edu.

Received July 31, 2007; accepted September 28, 2007; published October 11, 2007.

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