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First published online January 11, 2008; 10.1104/pp.107.112185

Plant Physiology 146:916-926 (2008)
© 2008 American Society of Plant Biologists

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Indole-3-Acetonitrile Production from Indole Glucosinolates Deters Oviposition by Pieris rapae1,[W],[OA]

Martin de Vos, Ksenia L. Kriksunov and Georg Jander*

Boyce Thompson Institute for Plant Research, Ithaca, New York 14853

Like many crucifer-specialist herbivores, Pieris rapae uses the presence of glucosinolates as a signal for oviposition and larval feeding. Arabidopsis thaliana glucosinolate-related mutants provide a unique resource for studying the in vivo role of these compounds in affecting P. rapae oviposition. Low indole glucosinolate cyp79B2 cyp79B3 mutants received fewer eggs than wild type, confirming prior research showing that indole glucosinolates are an important oviposition cue. Transgenic plants overexpressing epithiospecifier protein, which shifts glucosinolate breakdown toward nitrile formation, are less attractive to ovipositing P. rapae females. Exogenous application of indol-3-ylmethylglucosinolate breakdown products to cyp79B2 cyp79B3 mutants showed that oviposition was increased by indole-3-carbinol and decreased by indole-3-acetonitrile (IAN). P. rapae larvae tolerate a cruciferous diet by using a gut enzyme to redirect glucosinolate breakdown toward less toxic nitriles, including IAN, rather than isothiocyanates. The presence of IAN in larval regurgitant contributes to reduced oviposition by adult females on larvae-infested plants. Therefore, production of nitriles via epithiospecifier protein in cruciferous plants, which makes the plants more sensitive to generalist herbivores, may be a counter-adaptive mechanism for reducing oviposition by P. rapae and perhaps other crucifer-specialist insects.

1 This work was supported by the National Science Foundation (grant nos. IOS–0718733 and DBI–0500550 to G.J.), by the Netherlands Organisation for Scientific Research (award to M.d.V.), and by a fellowship to K.L.K. from C. Sampson.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Georg Jander (gj32{at}cornell.edu).

[W] The online version of this article contains Web-only data.

[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.112185

* Corresponding author; e-mail gj32{at}cornell.edu.

Received October 31, 2007; accepted December 31, 2007; published January 11, 2008.

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