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First published online January 30, 2008; 10.1104/pp.107.110379

Plant Physiology 146:1622-1636 (2008)
© 2008 American Society of Plant Biologists

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DEVELOPMENT AND HORMONE ACTION

The Transcription Factor MtSERF1 of the ERF Subfamily Identified by Transcriptional Profiling Is Required for Somatic Embryogenesis Induced by Auxin Plus Cytokinin in Medicago truncatula1,[W],[OA]

Feky R. Mantiri2, Sergey Kurdyukov2, Dasharath P. Lohar, Natalya Sharopova, Nasir A. Saeed, Xin-Ding Wang, Kathryn A. VandenBosch and Ray J. Rose*

Australian Research Council Centre of Excellence for Integrative Legume Research, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (F.R.M., S.K., N.A.S., X.-D.W., R.J.R.); and Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108 (D.P.L., N.S., K.A.V.)

Transcriptional profiling of embryogenic callus produced from Medicago truncatula mesophyll protoplasts indicated up-regulation of ethylene biosynthesis and ethylene response genes. Using inhibitors of ethylene biosynthesis and perception, it was shown that ethylene was necessary for somatic embryogenesis (SE) in this model legume. We chose several genes involved in ethylene biosynthesis and response for subsequent molecular analyses. One of these genes is a gene encoding a transcription factor that belongs to the AP2/ERF superfamily and ERF subfamily of transcription factors. We demonstrate that this gene, designated M. truncatula SOMATIC EMBRYO RELATED FACTOR1 (MtSERF1), is induced by ethylene and is expressed in embryogenic calli. MtSERF1 is strongly expressed in the globular somatic embryo and there is high expression in a small group of cells in the developing shoot meristem of the heart-stage embryo. RNA interference knockdown of this gene causes strong inhibition of SE. We also provide evidence that MtSERF1 is expressed in zygotic embryos. MtSERF1 appears to be essential for SE and may enable a connection between stress and development.

1 This work was supported in part by the Australian Research Council Centre of Excellence (grant no. CEO348212) to the University of Newcastle Node of the Centre of Excellence for Integrative Legume Research. Support for microarray analysis was provided by the National Science Foundation Plant Genome project (grant no. 0110206) and the University of Minnesota.

2 These authors contributed equally to the article.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Ray J. Rose (ray.rose{at}newcastle.edu.au).

[W] The online version of this article contains Web-only data.

[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.110379

* Corresponding author; e-mail ray.rose{at}newcastle.edu.au.

Received October 4, 2007; accepted January 16, 2008; published January 30, 2008.

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