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WHOLE PLANT AND ECOPHYSIOLOGY

Nitrogen Recycling and Remobilization Are Differentially Controlled by Leaf Senescence and Development Stage in Arabidopsis under Low Nitrogen Nutrition¹

Unité de Nutrition Azotée des Plantes, UR511, INRA, F–78000 Versailles, France (C.D., T.L., A.C., M.A., J.-F.M.-G., C.M.-D.); Division of Integrated Life Science, Graduate School of Biostudies, Kyoto 606–8502, Japan (F.S.); and UMR INRA-UCBN, Ecophysiologie Végétale, Agronomie et Nutritions, Université de Caen, F–14000 Caen, France (F.L.D.)

Five recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana), previously selected from the Bay-0 x Shahdara RIL population on the basis of differential leaf senescence phenotypes (from early senescing to late senescing) when cultivated under nitrogen (N)-limiting conditions, were analyzed to monitor metabolic markers related to N assimilation and N remobilization pathways. In each RIL, a decrease of total N, free amino acid, and soluble protein contents with leaf aging was observed. In parallel, the expression of markers for N remobilization such as cytosolic glutamine synthetase, glutamate dehydrogenase, and CND41-like protease was increased. This increase occurred earlier and more rapidly in early-senescing lines than in late-senescing lines. We measured the partitioning of ¹⁵N between sink and source leaves during the vegetative stage of development using ¹⁵N tracing and showed that N remobilization from the source leaves to the sink leaves was more efficient in the early-senescing lines. The N remobilization rate was correlated with leaf senescence severity at the vegetative stage. Experiments of ¹⁵N tracing at the reproductive stage showed, however, that the rate of N remobilization from the rosettes to the flowering organs and to the seeds was similar in early- and late-senescing lines. At the reproductive stage, N remobilization efficiency did not depend on senescence phenotypes but was related to the ratio between the biomasses of the sink and the source organs.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Céline Masclaux-Daubresse (masclaux{at}versailles.inra.fr).

www.plantphysiol.org/cgi/doi/10.1104/pp.108.119040

^* Corresponding author; e-mail masclaux{at}versailles.inra.fr.

Received March 17, 2008; accepted April 26, 2008; published May 8, 2008.

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