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Synthetic Lipid (DOPG) Vesicles Accumulate in the Cell Plate Region But Do Not Fuse^1,[W],[OA]

Laboratory of Plant Cell Biology, Department of Plant Sciences, Wageningen University, 6703–BD Wageningen, The Netherlands (A.E.-O., J.W.V., A.A.M.v.L., A.M.C.E.); and FOM Institute for Atomic and Molecular Physics, 1009–DB Amsterdam, The Netherlands (A.M.C.E.)

The cell plate is the new cell wall, with bordering plasma membrane, that is formed between two daughter cells in plants, and it is formed by fusion of vesicles (approximately 60 nm). To start to determine physical properties of cell plate forming vesicles for their transport through the phragmoplast, and fusion with each other, we microinjected fluorescent synthetic lipid vesicles that were made of 1,2-dioleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DOPG) into Tradescantia virginiana stamen hair cells. During interphase, the 60-nm wide DOPG vesicles moved inside the cytoplasm comparably to organelles. During cytokinesis, they were transported through the phragmoplast and accumulated in the cell plate region together with the endogenous vesicles, even inside the central cell plate region. Because at this stage microtubules are virtually absent from that region, while actin filaments are present, actin filaments may have a role in the transport of vesicles toward the cell plate. Unlike the endogenous vesicles, the synthetic DOPG vesicles did not fuse with the developing cell plate. Instead, they redistributed into the cytoplasm of the daughter cells upon completion of cytokinesis. Because the redistribution of the vesicles occurs when actin filaments disappear from the phragmoplast, actin filaments may be involved in keeping the vesicles inside the developing cell plate region.

² Present address: Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Geert Grooteplein zuid 28, 6500–HB Nijmegen, The Netherlands.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Anne Mie C. Emons (annemie.emons{at}wur.nl).

^[W] The online version of this article contains Web-only data.

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www.plantphysiol.org/cgi/doi/10.1104/pp.108.119842

^* Corresponding author; e-mail annemie.emons{at}wur.nl.

Received March 26, 2008; accepted June 19, 2008; published June 26, 2008.

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