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DEVELOPMENT AND HORMONE ACTION

The Arabidopsis Phosphatidylinositol 3-Kinase Is Important for Pollen Development^1,[W],[OA]

POSTECH-UZH Global Research Laboratory, Division of Molecular Life Sciences, POSTECH, Pohang 790–784, Korea (Yu.L., Y.C., Yo.L.); Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660–701, Korea (Yo.L.); School of Life Sciences and Biotechnology, Korea University, Seoul 136–701, Korea (E.-S.K., Y.-Y.C.); Center for Plant Intracellular Trafficking, POSTECH, Pohang 790–784, Korea (I.H.); and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907–1392 (C.J.S.)

Phosphatidylinositol 3-kinase has been reported to be important for normal plant growth. To characterize the role of the enzyme further, we attempted to isolate Arabidopsis (Arabidopsis thaliana) plants that do not express the gene, but we could not recover homozygous mutant plants. The progeny of VPS34/vps34 heterozygous plants, harboring a T-DNA insertion, showed a segregation ratio of 1:1:0 for wild-type, heterozygous, and homozygous mutant plants, indicating a gametophytic defect. Genetic transmission analysis showed that the abnormal segregation ratio was due to failure to transmit the mutant allele through the male gametophyte. Microscopic observation revealed that 2-fold higher proportions of pollen grains in heterozygous plants than wild-type plants were dead or showed reduced numbers of nuclei. Many mature pollen grains from the heterozygous plants contained large vacuoles even until the mature pollen stage, whereas pollen from wild-type plants contained many small vacuoles beginning from the vacuolated pollen stage, which indicated that vacuoles in many of the heterozygous mutant pollen did not undergo normal fission after the first mitotic division. Taken together, our results suggest that phosphatidylinositol 3-kinase is essential for vacuole reorganization and nuclear division during pollen development.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Youngsook Lee (ylee{at}postech.ac.kr).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.121590

^* Corresponding author; e-mail ylee{at}postech.ac.kr.

Received April 22, 2008; accepted May 2, 2008; published May 30, 2008.

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